Sulfo-TAG NHS ester disodium

Name: Sulfo-TAG NHS ester disodium
Brand: MedChemExpress
SKU: HY-153631
Rating: 4.5 (1 reviews)

Cat. No.: HY-153631: Data Sheet
SDS
Handling Instructions
FAQs
Technical Support

Sulfo-TAG NHS ester disodium is an N-hydroxysuccinimide ester that can be coupled to proteins via primary amine groups. Sulfo-TAG NHS ester disodium can be used for antibody labeling.

For research use only. We do not sell to patients.

Sulfo-TAG NHS ester disodium Chemical Structure

CAS No. : 482618-42-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	Get quote	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Sulfo-TAG NHS ester disodium:

Sulfo-TAG TFP ester disodium Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Sulfo-TAG NHS ester disodium is an N-hydroxysuccinimide ester that can be coupled to proteins via primary amine groups. Sulfo-TAG NHS ester disodium can be used for antibody labeling^[1].

In Vitro

Guide (The following are our recommended protocols. This protocol is only a guideline and should be modified according to your specific needs).
Antibody Labeling Procedure^[2]
1. For lyophilized antibodies, reconstitute with PBS buffer; for antibodies in solution, replace the antibody with PBS buffer at pH 7.9 using a Zeba desalting column with a molecular weight cutoff of 7000 Daltons.
2. Determine the antibody concentration.
3. Add 3 nmol/μL of Sulfo-Tag NHS-Ester to the antibody and mix at room temperature in the dark for 2 h.
4. Replace the antibody with PBS buffer at pH 7.4/0.05% sodium azide using a Zeba desalting column.
5. Measure the absorbance of the protein conjugate at 455 nm using a NanoDrop.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1185.12

Formula

C₄₃H₃₉N₇Na₂O₁₆RuS₄

CAS No.

482618-42-8

Appearance

Solid

Color

Orange to red

SMILES

O=[S](CC1=CC=[N](C2=C1)[Ru+2]([N](C3=C4)=CC=C4C[S](=O)(O[Na])=O)([N](C2=C5)=CC=C5C[S](=O)([O-])=O)([N](C6=C7)=CC=C7CCCC(ON(C8=O)C(CC8)=O)=O)([N](C6=C9)=CC=C9C)[N](C3=C%10)=CC=C%10C[S](=O)(O[Na])=O)([O-])=O

Shipping

Shipping with dry ice.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (84.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (84.38 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8438 mL	4.2190 mL	8.4380 mL
5 mM	0.1688 mL	0.8438 mL	1.6876 mL
10 mM	0.0844 mL	0.4219 mL	0.8438 mL

View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Data Sheet (281 KB) SDS (480 KB) Handling Instructions (2659 KB)

References

[1]. Kanghoon Lee, et al. Analysis of ocular fluid in patients with ranibizumab-recalcitrant neovascular age-related macular degeneration who have serum anti-ranibizumab antibodies. Graefes Arch Clin Exp Ophthalmol. 2023, 261, 12. [Content Brief]

[2]. Pang S, et al. A pilot study to evaluate the application of a generic protein standard panel for quality control of biomarker detection technologies. BMC Res Notes. 2011 Aug 11;4:281. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	0.8438 mL	4.2190 mL	8.4380 mL	21.0949 mL
	5 mM	0.1688 mL	0.8438 mL	1.6876 mL	4.2190 mL
	10 mM	0.0844 mL	0.4219 mL	0.8438 mL	2.1095 mL
	15 mM	0.0563 mL	0.2813 mL	0.5625 mL	1.4063 mL
	20 mM	0.0422 mL	0.2109 mL	0.4219 mL	1.0547 mL
	25 mM	0.0338 mL	0.1688 mL	0.3375 mL	0.8438 mL
	30 mM	0.0281 mL	0.1406 mL	0.2813 mL	0.7032 mL
	40 mM	0.0211 mL	0.1055 mL	0.2109 mL	0.5274 mL
	50 mM	0.0169 mL	0.0844 mL	0.1688 mL	0.4219 mL
	60 mM	0.0141 mL	0.0703 mL	0.1406 mL	0.3516 mL
	80 mM	0.0105 mL	0.0527 mL	0.1055 mL	0.2637 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.