Sulfo-TAG NHS ester disodium
Sulfo-TAG NHS ester disodium is an N-hydroxysuccinimide ester that can be coupled to proteins via primary amine groups. Sulfo-TAG NHS ester disodium can be used for antibody labeling.
For research use only. We do not sell to patients.
- CAS No.: 482618-42-8
- Formula: C43H39N7Na2O16RuS4
- Molecular Weight:1185.12
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following are our recommended protocols. This protocol is only a guideline and should be modified according to your specific needs).
Antibody Labeling Procedure[2]
1. For lyophilized antibodies, reconstitute with PBS buffer; for antibodies in solution, replace the antibody with PBS buffer at pH 7.9 using a Zeba desalting column with a molecular weight cutoff of 7000 Daltons.
2. Determine the antibody concentration.
3. Add 3 nmol/μL of Sulfo-Tag NHS-Ester to the antibody and mix at room temperature in the dark for 2 h.
4. Replace the antibody with PBS buffer at pH 7.4/0.05% sodium azide using a Zeba desalting column.
5. Measure the absorbance of the protein conjugate at 455 nm using a NanoDrop.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 482618-42-8
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Appearance Solid
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Molecular Weight 1185.12
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Formula C43H39N7Na2O16RuS4
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Color Orange to red
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SMILES
O=[S](CC1=CC=[N](C2=C1)[Ru+2]([N](C3=C4)=CC=C4C[S](=O)(O[Na])=O)([N](C2=C5)=CC=C5C[S](=O)([O-])=O)([N](C6=C7)=CC=C7CCCC(ON(C8=O)C(CC8)=O)=O)([N](C6=C9)=CC=C9C)[N](C3=C%10)=CC=C%10C[S](=O)(O[Na])=O)([O-])=O
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Shipping
Shipping with dry ice.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 100 mg/mL (84.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (84.38 mM)
* "≥" means soluble, but saturation unknown.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Kanghoon Lee, et al. Analysis of ocular fluid in patients with ranibizumab-recalcitrant neovascular age-related macular degeneration who have serum anti-ranibizumab antibodies. Graefes Arch Clin Exp Ophthalmol. 2023, 261, 12. [Content Brief]
[2]. Pang S, et al. A pilot study to evaluate the application of a generic protein standard panel for quality control of biomarker detection technologies. BMC Res Notes. 2011 Aug 11;4:281. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.8438 mL | 4.2190 mL | 8.4380 mL | 21.0949 mL |
| 5 mM | 0.1688 mL | 0.8438 mL | 1.6876 mL | 4.2190 mL | |
| 10 mM | 0.0844 mL | 0.4219 mL | 0.8438 mL | 2.1095 mL | |
| 15 mM | 0.0563 mL | 0.2813 mL | 0.5625 mL | 1.4063 mL | |
| 20 mM | 0.0422 mL | 0.2109 mL | 0.4219 mL | 1.0547 mL | |
| 25 mM | 0.0338 mL | 0.1688 mL | 0.3375 mL | 0.8438 mL | |
| 30 mM | 0.0281 mL | 0.1406 mL | 0.2813 mL | 0.7032 mL | |
| 40 mM | 0.0211 mL | 0.1055 mL | 0.2109 mL | 0.5274 mL | |
| 50 mM | 0.0169 mL | 0.0844 mL | 0.1688 mL | 0.4219 mL | |
| 60 mM | 0.0141 mL | 0.0703 mL | 0.1406 mL | 0.3516 mL | |
| 80 mM | 0.0105 mL | 0.0527 mL | 0.1055 mL | 0.2637 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Sulfo-TAG NHS ester disodium Related Classifications
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