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Sulfo-TAG NHS ester disodium is an N-hydroxysuccinimide ester that can be coupled to proteins via primary amine groups. Sulfo-TAG NHS ester disodium can be used for antibody labeling.

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Sulfo-TAG NHS ester disodium

Sulfo-TAG NHS ester disodium 構造式

CAS 番号 : 482618-42-8

容量 価格(税別) 在庫状況 数量
1 mg $592 在庫あり
5 mg $1184 お問い合わせ
10 mg $1777 お問い合わせ
50 mg   お問い合わせ  
100 mg   お問い合わせ  

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製品説明

Sulfo-TAG NHS ester disodium is an N-hydroxysuccinimide ester that can be coupled to proteins via primary amine groups. Sulfo-TAG NHS ester disodium can be used for antibody labeling[1].

体外実験

Guide (The following are our recommended protocols. This protocol is only a guideline and should be modified according to your specific needs).
Antibody Labeling Procedure[2]
1. For lyophilized antibodies, reconstitute with PBS buffer; for antibodies in solution, replace the antibody with PBS buffer at pH 7.9 using a Zeba desalting column with a molecular weight cutoff of 7000 Daltons.
2. Determine the antibody concentration.
3. Add 3 nmol/μL of Sulfo-Tag NHS-Ester to the antibody and mix at room temperature in the dark for 2 h.
4. Replace the antibody with PBS buffer at pH 7.4/0.05% sodium azide using a Zeba desalting column.
5. Measure the absorbance of the protein conjugate at 455 nm using a NanoDrop.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1185.12

分子式

C43H39N7Na2O16RuS4

CAS 番号
Appearance

Solid

Color

Orange to red

SMILES

O=[S](CC1=CC=[N](C2=C1)[Ru+2]([N](C3=C4)=CC=C4C[S](=O)(O[Na])=O)([N](C2=C5)=CC=C5C[S](=O)([O-])=O)([N](C6=C7)=CC=C7CCCC(ON(C8=O)C(CC8)=O)=O)([N](C6=C9)=CC=C9C)[N](C3=C%10)=CC=C%10C[S](=O)(O[Na])=O)([O-])=O

輸送条件

Shipping with dry ice.

保管条件

-80°C, sealed storage, away from moisture and light

溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (84.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 100 mg/mL (84.38 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8438 mL 4.2190 mL 8.4380 mL
5 mM 0.1688 mL 0.8438 mL 1.6876 mL
10 mM 0.0844 mL 0.4219 mL 0.8438 mL
View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます:C1V1 = C2V2

濃度 (開始)

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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参考文献

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 0.8438 mL 4.2190 mL 8.4380 mL 21.0949 mL
5 mM 0.1688 mL 0.8438 mL 1.6876 mL 4.2190 mL
10 mM 0.0844 mL 0.4219 mL 0.8438 mL 2.1095 mL
15 mM 0.0563 mL 0.2813 mL 0.5625 mL 1.4063 mL
20 mM 0.0422 mL 0.2109 mL 0.4219 mL 1.0547 mL
25 mM 0.0338 mL 0.1688 mL 0.3375 mL 0.8438 mL
30 mM 0.0281 mL 0.1406 mL 0.2813 mL 0.7032 mL
40 mM 0.0211 mL 0.1055 mL 0.2109 mL 0.5274 mL
50 mM 0.0169 mL 0.0844 mL 0.1688 mL 0.4219 mL
60 mM 0.0141 mL 0.0703 mL 0.1406 mL 0.3516 mL
80 mM 0.0105 mL 0.0527 mL 0.1055 mL 0.2637 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます:C1V1 = C2V2

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

製品名:
Sulfo-TAG NHS ester disodium
製品番号:
HY-153631
数量:
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