Cat. No.: HY-N2450

Sulforaphene

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Sulforaphene, isolated from radish seeds, exhibits an ED₅₀ against velvetleaf seedlings approximately 2 x 10^-4 M. Sulforaphene promotes cancer cells apoptosis and inhibits migration via inhibiting EGFR, p-ERK1/2, NF‐κB and other signals.

For research use only. We do not sell to patients.

Purity: 99.78%
CAS No.: 592-95-0
Formula: C₆H₉NOS₂
Molecular Weight:175.27

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Sulforaphene Chemical Structure

CAS No.: 592-95-0

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All EGFR Isoforms :

All ERK Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	18.74 μM Compound: SFE	Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
A549	IC₅₀	9.33 μM Compound: SFE	Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
HCT-116	IC₅₀	31.92 μM Compound: SFE	Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
HeLa	IC₅₀	85.05 μM Compound: SFE	Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay	[PMID: 30282324]
SMMC-7721	IC₅₀	6.41 μM Compound: SFE	Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay	[PMID: 30282324]

In Vitro

Sulforaphene (0-80 μM, 48 h) inhibits HCC (HepG2 and Hep3B cells) cell viability and induces apoptosis^[4].
Sulforaphene (0-40 μM) sensitizes radiosensitivity of HepG2 and Hep3B cells, and promotes radiation-induced cell death^[4].
Sulforaphene (0-10 μM, 24 h) inhibits SUM159 cell migration and invasion by inhibiting Hedgehog signaling^[5].
Sulforaphene (5 μM, 24 h) inhibits microtubule polymerization in HCT116 and HT-29 cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[5]

Cell Line:

HepG2 and Hep3B cell
Concentration:

0-40 μM
Incubation Time:

48 h
Result:

Increased Bax expression and reduced Bcl-2 expression.
Inhibited expression of NF-𝜅B-dependent genes (COX-2, iNos, and cyclinD1).

In Vivo

Sulforaphene (1 and 5 mg/kg, i.p., everyday) inhibits tumor growth in a mouse HCT116 xenograft model^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse HCT116 xenograft model^[6]
Dosage:

1 and 5 mg/kg
Administration:

i.p., everyday
Result:

Inhibited tumor growth, and increased CDK1, MK2, and p38 phosphorylation.

Chemical Information

CAS No. 592-95-0
Appearance Liquid (Density: 1.16±0.1 g/cm³)
Molecular Weight 175.27
Formula C₆H₉NOS₂
Color Colorless to light yellow
SMILES

O=S(/C=C/CCN=C=S)C
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

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Journal Impact Factor

Cell Rep

Shear stress-induced Piezo1 activates CD99L2 to facilitate the initiation of blood circulation. [Abstract]

2026 Mar 30;45(4):117200. PMID: 41915472
J Evid Based Integr Med

Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α. [Abstract]

2025 Jan-Dec:30:2515690X251316760. PMID: 39905916

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (570.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7055 mL

28.5274 mL

57.0548 mL

5 mM

1.1411 mL

5.7055 mL

11.4110 mL

10 mM

0.5705 mL

2.8527 mL

5.7055 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kuang P, et al. Separation and purification of sulforaphene from radish seeds using macroporous resin and preparative high-performance liquid chromatography. Food Chem. 2013 Jan 15;136(2):342-7. [Content Brief]

[2]. Anita M. Brinker, et al. Herbicidal activity of sulforaphene from stock (Matthiola incana). Journal of Chemical Ecology. Vol. 19. No. 10, 1993.

[3]. Mondal A, et al. Sulforaphene promotes Bax/Bcl2, MAPK-dependent human gastric cancer AGS cells apoptosis and inhibits migration via EGFR, p-ERK1/2 down-regulation. General Physiology and Biophysics, 27 Nov 2015, 35(1):25-34.

[4]. Ren K, et al. Sulforaphene enhances radiosensitivity of hepatocellular carcinoma through suppression of the NF-κB pathway. J Biochem Mol Toxicol. 2017 Aug;31(8). [Content Brief]

[5]. Bao C, et al. Sulforaphene Interferes with Human Breast Cancer Cell Migration and Invasion through Inhibition of Hedgehog Signaling. J Agric Food Chem. 2016 Jul 13;64(27):5515-24. [Content Brief]

[6]. Byun S, et al. Sulforaphene suppresses growth of colon cancer-derived tumors via induction of glutathione depletion and microtubule depolymerization. Mol Nutr Food Res. 2016 May;60(5):1068-78. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.7055 mL	28.5274 mL	57.0548 mL	142.6371 mL
	5 mM	1.1411 mL	5.7055 mL	11.4110 mL	28.5274 mL
	10 mM	0.5705 mL	2.8527 mL	5.7055 mL	14.2637 mL
	15 mM	0.3804 mL	1.9018 mL	3.8037 mL	9.5091 mL
	20 mM	0.2853 mL	1.4264 mL	2.8527 mL	7.1319 mL
	25 mM	0.2282 mL	1.1411 mL	2.2822 mL	5.7055 mL
	30 mM	0.1902 mL	0.9509 mL	1.9018 mL	4.7546 mL
	40 mM	0.1426 mL	0.7132 mL	1.4264 mL	3.5659 mL
	50 mM	0.1141 mL	0.5705 mL	1.1411 mL	2.8527 mL
	60 mM	0.0951 mL	0.4755 mL	0.9509 mL	2.3773 mL
	80 mM	0.0713 mL	0.3566 mL	0.7132 mL	1.7830 mL
	100 mM	0.0571 mL	0.2853 mL	0.5705 mL	1.4264 mL

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Sulforaphene

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Publications Citing Use of MedChemExpress (MCE) Sulforaphene

All EGFR Isoforms