W-7 hydrochloride
Based on 7 publication(s) in Google Scholar
W-7 hydrochloride is a selective calmodulin antagonist. W-7 hydrochloride inhibits the Ca2+-calmodulin-dependent phosphodiesterase and myosin light chain kinase with IC50 values of 28 μM and 51 μM, respectively. W-7 hydrochloride induces apoptosis and has antitumor and vascular relaxing activity. W-7 hydrochloride is a blocker of Kv4.3 and can be used for research of arrhythmias.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 61714-27-0
- Formula: C16H22Cl2N2O2S
- Molecular Weight:377.33
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) W-7 hydrochloride
More- Mol Cell. 2025 Oct 29:S1097-2765(25)00822-6. [Abstract]
- Adv Healthc Mater. 2023 Dec;12(31):e2301137. [Abstract]
- Int J Biol Macromol. 2024 Aug 3:134478. [Abstract]
- FASEB J. 2025 Dec 31;39(24):e71322. [Abstract]
- J Biol Chem. 2024 Jun;300(6):107348. [Abstract]
- Sensors (Basel). 2024 Oct 26;24(21):6869. [Abstract]
- J Cancer Res Clin Oncol. 2024 Sep 19;150(9):424. [Abstract]
Customer Validation & Images
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WB
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IF
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Cell Proliferation/Viability Assay
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Cell Migration/Invasion Assay
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RT-PCR
Biological Activity
IC50: 28 μM (Phosphodiesterase) and 51 µM (Myosin light chain kinase)[1]
W-7 hydrochloride is distributed mainly in the cytoplasm, and inhibits proliferation of Chinese hamster ovary K1 (CHO-K1) cells. W-7 hydrochloride selectively blocks the phase of the cell cycle (G1/S boundary phase) in a manner. 25 μM W-7 hydrochloride arrests the growth of the cells at the G1/S boundary phase of the cell cycle[1].
W-7 (100 μM) hydrochloride exhibits a similar extent of antagonism between the contractile responses to carbachol and KCl. The increase in myosin light chain (P-LC) phosphate content in response to 1-min stimulation with 10 μM carbachol is inhibited by W-7 hydrochloride. W-7 hydrochloride antagonizes the smooth muscle contraction through the inhibition of the initial increase in the P-LC phosphorylation[2].
Treatment with W-7 hydrochloride results in the dose-dependent inhibition of cell proliferation in various human multiple myeloma cell lines. W-7 hydrochloride induces G1 phase cell cycle arrest by downregulating cyclins and upregulating p21cip1. W-7 hydrochloride induces apoptosis via caspase activation; this occurred partly through the elevation of intracellular calcium levels and mitochondrial membrane potential depolarization and through inhibition of the STAT3 phosphorylation and subsequent downregulation of Mcl-1 protein[3].
W-7 hydrochloride competitively inhibits Ca2+/calmodulin-dependent phosphodiesterase with a Ki value of 300 μM[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c nu mice (6-week-old) injected with RPMI 8226 cells[3]
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Dosage:3 mg/kg
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Administration:Intraperitoneal injection; on 5 consecutive days per week
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Result:Significantly reduced tumor growth in a murine MM model.
Chemical Information
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CAS No. 61714-27-0
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Appearance Solid
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Molecular Weight 377.33
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Formula C16H22Cl2N2O2S
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Color Off-white to yellow
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SMILES
O=S(C1=C2C=CC=C(Cl)C2=CC=C1)(NCCCCCCN)=O.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Mol Cell
The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells. [Abstract]2025 Oct 29:S1097-2765(25)00822-6. PMID: 41167191
W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 29:S1097-2765(25)00822-6. [Abstract]
HEK293T cells expressing FLAG‑tagged RNF32 were treated with PMA and IO for 90 min, then immunoprecipitated (IP) with an anti‑RNF32 antibody in the presence or absence of the indicated CaM inhibitors W-7 hydrochloride (25-100 μM) or Calmidazolium. Whole cell extracts (WCEs) were immunoprecipitated with anti-FLAG resin. Immunocomplexes and WCEs were analyzed by immunoblotting. The results showed that the CaM inhibitors W-7 hydrochloride and Calmidazolium reduced the binding of RNF32 to the IKK complex in cells treated with PMA and IO.
W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 29:S1097-2765(25)00822-6. [Abstract]
Immunofluorescence staining of endogenous RNF32 in U2OS cells stimulated with 10 ng/ml phorbol-12-myristate-13-acetate (PMA) and 950 nM ionomycin (IO) for 90 minutes with or without the CaM inhibitor W‑7 hydrochloride (25-100 μM). The results showed that the CaM inhibitors W‑7 hydrochloride greatly reduced the formation of RNF32 condensates. Scale bars indicate 10 µm.
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Adv Healthc Mater
2023 Dec;12(31):e2301137. PMID: 37671812 -
Int J Biol Macromol
Nanoparticle-mediated calmodulin dsRNA and cyantraniliprole co-delivery system: High-efficient control of two key pear pests while ensuring safety for natural enemy insects. [Abstract]2024 Aug 3:134478. PMID: 39102908
W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Aug 3:134478. [Abstract]
The relative expression levels of CaM and CN genes following inhibitor treatment at 48 h. The results showed that 10 μM W-7 hydrochloride (48 h) significantly downregulated CaM genes in both insects, while FK506 exhibited superior efficacy at 100 μM.
W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Aug 3:134478. [Abstract]
The survival rate of G. molesta and C. chinensis after treatment with inhibitors and cyantraniliprole (CY). The results showed that injection or oral administration of W-7 hydrochloride (10 μM) or FK506 (100 μM) increased the sensitivity of both G. molesta and C. chinensis to cyantraniliprole (LC20), resulting in a notable rise in mortality rates, with the cyantraniliprole/W7 group showing the highest mortality.
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FASEB J
ORAI3 Modulates Oral Squamous Cell Carcinoma Metastasis Through the Ca2+/Calmodulin/Calcineurin/ETV4 Signaling Pathway. [Abstract]2025 Dec 31;39(24):e71322. PMID: 41417281
W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Dec 31;39(24):e71322. [Abstract]
Results of CCK-8 assays assessing the proliferation of OSCC cells (Cal-27, HN4) in the sgNC and sgORAI3 groups, as well as following treatment with inhibitors W-7 hydrochloride (10 μM) for calmodulin and CsA for calcineurin. Fold changes are relative to the optical density at a wavelength of 450 nm at time 0 h.
W-7 hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Dec 31;39(24):e71322. [Abstract]
Representative images of scratch assays showing the migration of OSCC cells in the sgNC and sgORAI3 groups, as well as following treatment with W-7 hydrochloride (10 μM; 24-48 h) and CsA. Scale bar, 200 μm.
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J Biol Chem
Calcium and IL-6 regulate the anterograde trafficking and plasma membrane residence of the iron exporter ferroportin to modulate iron efflux. [Abstract]2024 Jun;300(6):107348. PMID: 38718866 -
Sensors (Basel)
A Novel Single-Color FRET Sensor for Rho-Kinase Reveals Calcium-Dependent Activation of RhoA and ROCK. [Abstract]2024 Oct 26;24(21):6869. PMID: 39517770 -
J Cancer Res Clin Oncol
MYO3B promotes cancer progression in endometrial cancer by mediating the calcium ion-RhoA/ROCK1 signaling pathway. [Abstract]2024 Sep 19;150(9):424. PMID: 39297944
Solvent & Solubility
DMSO : 250 mg/mL (662.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1.43 mg/mL (3.79 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. H Hidaka, et al. N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a Calmodulin Antagonist, Inhibits Cell Proliferation. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4354-7. [Content Brief]
[2]. M Asano. Divergent Pharmacological Effects of Three Calmodulin Antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), Chlorpromazine and Calmidazolium, on Isometric Tension Development and Myosin Light Chain Phosphorylation in Intact Bovine Tracheal Smooth Muscle. J Pharmacol Exp Ther. 1989 Nov;251(2):764-73. [Content Brief]
[3]. H Itoh, et al. Direct Interaction of Calmodulin Antagonists With Ca2+/calmodulin-dependent Cyclic Nucleotide Phosphodiesterase. J Biochem. 1984 Dec;96(6):1721-6. [Content Brief]
[4]. Shigeyuki Yokokura, et al. Calmodulin Antagonists Induce Cell Cycle Arrest and Apoptosis in Vitro and Inhibit Tumor Growth in Vivo in Human Multiple Myeloma. BMC Cancer. 2014 Nov 26;14:882. [Content Brief]
[5]. Qu YJ, et al. W-7 modulates Kv4.3: pore block and Ca2+-calmodulin inhibition. Am J Physiol Heart Circ Physiol. 2007 May;292(5):H2364-77. [Content Brief]
[6]. Gbadebo TD, et al. Calmodulin inhibitor W-7 unmasks a novel electrocardiographic parameter that predicts initiation of torsade de pointes. Circulation. 2002 Feb 12;105(6):770-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.6502 mL | 13.2510 mL | 26.5020 mL | 66.2550 mL |
| DMSO | 5 mM | 0.5300 mL | 2.6502 mL | 5.3004 mL | 13.2510 mL |
| 10 mM | 0.2650 mL | 1.3251 mL | 2.6502 mL | 6.6255 mL | |
| 15 mM | 0.1767 mL | 0.8834 mL | 1.7668 mL | 4.4170 mL | |
| 20 mM | 0.1325 mL | 0.6626 mL | 1.3251 mL | 3.3128 mL | |
| 25 mM | 0.1060 mL | 0.5300 mL | 1.0601 mL | 2.6502 mL | |
| 30 mM | 0.0883 mL | 0.4417 mL | 0.8834 mL | 2.2085 mL | |
| 40 mM | 0.0663 mL | 0.3313 mL | 0.6626 mL | 1.6564 mL | |
| 50 mM | 0.0530 mL | 0.2650 mL | 0.5300 mL | 1.3251 mL | |
| 60 mM | 0.0442 mL | 0.2209 mL | 0.4417 mL | 1.1043 mL | |
| 80 mM | 0.0331 mL | 0.1656 mL | 0.3313 mL | 0.8282 mL | |
| 100 mM | 0.0265 mL | 0.1325 mL | 0.2650 mL | 0.6626 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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