2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis
  3. Reactive Oxygen Species (ROS) Endogenous Metabolite Apoptosis
  4. Zinc Protoporphyrin

Zinc Protoporphyrin  (Synonyms: Zn(II)-protoporphyrin IX; ZnPP; Zinc Protoporphyrin-9)

Cat. No.: HY-101193 Purity: 99.87%
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SDS
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Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) is an orally active and competitive heme oxygenase-1 (HO-1) inhibitor. Zinc Protoporphyrin regulates expression of HO-1 at the transcriptional level. The effect of Zinc Protoporphyrin on HO-1 expression is controversial.  It was shown to induce HO-1 expression in some cells, but suppress it in others. Zinc Protoporphyrin is used as a screening marker of iron deficiency in vivo. Zinc Protoporphyrin has anti-cancer activity.

For research use only. We do not sell to patients.

CAS No. : 15442-64-5

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Based on 67 publication(s) in Google Scholar

Other Forms of Zinc Protoporphyrin:

Zinc Protoporphyrin Related Antibodies

Top Publications Citing Use of Products

67 Publications Citing Use of MCE Zinc Protoporphyrin

Cell Proliferation/Viability Assay
Flow Cytometry
WB
Cell Imaging/Staining
Histological Imaging/Staining
In Vivo Efficacy Study

    Zinc Protoporphyrin purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 16;13(1):9.  [Abstract]

    MLO-Y4 cell viability after 48 h treatment with 75% B16F10 CM, with or without 24 h pretreatment with 10 μmol/L Fer-1, Znpp, or DMSO (control).

    Zinc Protoporphyrin purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 16;13(1):9.  [Abstract]

    FACS analysis using C11 BODIPY 581/591 and H2DCFDA to quantify ferroptosis in MLO-Y4 cells after 48 h treatment with 75% B16F10 CM, with or without 24 h pretreatment with 10 μmol/L Fer-1, Znpp, or DMSO (control).

    Zinc Protoporphyrin purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 16;13(1):9.  [Abstract]

    Western blot of MLO-Y4 cells after 48 h treatment with 75% B16F10 CM, with or without 24 h pretreatment with 10 μmol/L Znpp, or DMSO (control).

    Zinc Protoporphyrin purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 16;13(1):9.  [Abstract]

    Transmission electron microscopy (TEM) of MLO-Y4 cells after 48 h treatment with 75% B16F10 CM, with or without 24 h pretreatment with 10 μmol/L Znpp, or DMSO (control). Scale bars: 2500 nm (left), 1000 nm (right).

    Zinc Protoporphyrin purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 16;13(1):9.  [Abstract]

    H&E staining of cortical bones from mice injected with B16F10 and treated with DMSO, 1 mg/kg Fer-1, or 10 mg/kg Znpp (i.p., 13 d).

    Zinc Protoporphyrin purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 16;13(1):9.  [Abstract]

    µCT analysis of trabecular bone from mice injected with B16F10 and treated with DMSO, 1 mg/kg Fer-1, or 10 mg/kg Znpp (i.p., 13 d).

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) is an orally active and competitive heme oxygenase-1 (HO-1) inhibitor. Zinc Protoporphyrin regulates expression of HO-1 at the transcriptional level. The effect of Zinc Protoporphyrin on HO-1 expression is controversial.  It was shown to induce HO-1 expression in some cells, but suppress it in others. Zinc Protoporphyrin is used as a screening marker of iron deficiency in vivo. Zinc Protoporphyrin has anti-cancer activity[1][2][3][4][5].

    IC50 & Target[1][2]

    Human Endogenous Metabolite

     

    In Vitro

    to increase in Protoporphyrin (Zn(II)-protoporphyrin IX; 5 μM; 72 hours) causes the fraction of late apoptotic and necrotic cells increasing from 10.9% in controls to 30.4% after 72 h[3].
    Zinc Protoporphyrin (1.25-40 μM; 48 or 72 hours) exerts cytostatic/cytotoxic effects against tumor cells[3].
    Zinc Protoporphyrin (2.5, 5 μM; 48 or 72 hours) results in dose- and time-dependent reduction of cells in the G1 phase of the cell cycle[3].
    Zinc Protoporphyrin (1.25-40 μM; 48 hours) leads to the accumulation of cleaved (active) caspase-3[3].
    The effect of Zinc Protoporphyrin on HO-1 expression is controversial.  It was shown to induce HO-1 expression in some cells, but suppress it in others[4][5].
    For example, Zinc Protoporphyrin is shown to induce HO-1 expression in hamster fibroblast (HA-1) cells but not in Neuro-2A mouse neuroblastoma cells and primary cultures of rat cortical neurons.
    Zinc Protoporphyrin suppresses the induction of HO-1 by statins or lipopolysaccharide.
    Zinc Protoporphyrin induces HO-1 expression in human prostate adenocarcinoma PC-3 and breast adenocarcinoma MCF-7 cells.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Zinc Protoporphyrin Related Antibodies

    Apoptosis Analysis[3]

    Cell Line: C-26 cells
    Concentration: 5 μM
    Incubation Time: 72 hours
    Result: The fraction of late apoptotic and necrotic cells increased from 10.9% in controls to 30.4% after 72 h.

    Cell Cytotoxicity Assay[3]

    Cell Line: C-26 and MDA-MB231 cells
    Concentration: 1.25, 2.5, 5, 10, 20, 40 μM
    Incubation Time: 48 or 72 hours
    Result: Exerted cystostatic/cytotoxic effects against tumor cells.

    Cell Cycle Analysis[3]

    Cell Line: C-26 cells
    Concentration: 2.5, 5 μM
    Incubation Time: 48 or 72 hours
    Result: Resulted in dose- and time-dependent reduction of cells in G1 phase of the cell cycle.

    Western Blot Analysis[3]

    Cell Line: C-26 cells
    Concentration: 1.25, 2.5, 5, 10, 20, 40 μM
    Incubation Time: 48 hours
    Result: Leaded to accumulation of cleaved (active) caspase-3.
    In Vivo

    Zinc Protoporphyrin (12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.; from day 7 to 19) exerts dose-dependent antitumor effects manifested by the retardation of tumor growth[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c mice inoculated with C-26 cells[3]
    Dosage: 12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.
    Administration: IP or PO; from day 7 to 19
    Result: Exerted dose-dependent antitumor effects manifested by the retardation of tumor growth.
    Clinical Trial
    Molecular Weight

    626.02

    Formula

    C34H32N4O4Zn
    CAS No.
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    OC(CCC1=C(C=C2[N]3=C4C(C)=C2CCC(O)=O)[N-]([Zn+2]35[N]6=C7C=C(C(C=C)=C8C)[N-]5C8=C4)C(C=C6C(C=C)=C7C)=C1C)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 20.59 mg/mL (32.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5974 mL 7.9870 mL 15.9739 mL
    5 mM 0.3195 mL 1.5974 mL 3.1948 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 1.67 mg/mL (2.67 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.87%

    SDS (393 KB)

    COA (259 KB) HNMR (270 KB) RP-HPLC (256 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5974 mL 7.9870 mL 15.9739 mL 39.9348 mL
    5 mM 0.3195 mL 1.5974 mL 3.1948 mL 7.9870 mL
    10 mM 0.1597 mL 0.7987 mL 1.5974 mL 3.9935 mL
    15 mM 0.1065 mL 0.5325 mL 1.0649 mL 2.6623 mL
    20 mM 0.0799 mL 0.3993 mL 0.7987 mL 1.9967 mL
    25 mM 0.0639 mL 0.3195 mL 0.6390 mL 1.5974 mL
    30 mM 0.0532 mL 0.2662 mL 0.5325 mL 1.3312 mL
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    Zinc Protoporphyrin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Zinc Protoporphyrin15442-64-5Zn(II)-protoporphyrin IX ZnPP Zinc Protoporphyrin-9Zinc Protoporphyrin9Zinc Protoporphyrin 9Zinc Protoporphyrin-9Reactive Oxygen Species (ROS)Endogenous MetaboliteApoptosishemeoxygenase-1HO-1PhloroglucinolH2O2screeningmarkerirondeficiency,anti-cancerInhibitorinhibitorinhibit

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