1. Anti-infection
    Epigenetics
  2. HIV
    Epigenetic Reader Domain
  3. ZL0580

ZL0580 

Cat. No.: HY-126428 Purity: 99.48%
Handling Instructions

ZL0580, a structurally close analog of ZL0590, induces epigenetic suppression of HIV via selectively binding to BD1 domain of BRD4. ZL0580 induces HIV suppression by inhibiting Tat transactivation and transcription elongation as well as by inducing repressive chromatin structure at the HIV promoter.

For research use only. We do not sell to patients.

ZL0580 Chemical Structure

ZL0580 Chemical Structure

CAS No. : 2377151-10-3

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 410 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 580 In-stock
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50 mg USD 1550 In-stock
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100 mg USD 2400 In-stock
Estimated Time of Arrival: December 31
250 mg USD 3600 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

ZL0580, a structurally close analog of ZL0590, induces epigenetic suppression of HIV via selectively binding to BD1 domain of BRD4. ZL0580 induces HIV suppression by inhibiting Tat transactivation and transcription elongation as well as by inducing repressive chromatin structure at the HIV promoter[1][2][3].

IC50 & Target

BRD4 (BD1)

 

In Vitro

ZL0580 (8 μM, 2 days, PBMCs of viremic HIV-infected individuals) induces HIV transcriptional suppression with low toxicity[1].
ZL0580 treatment (10 μM) suppresses both PMA-stimulated and basal HIV transcription[1].

Cell Viability Assay[1]

Cell Line: HIV-infected human CD4+ T cells.
Concentration: 0-8 μM.
Incubation Time: 2 days.
Result: Suppress HIV in primary CD4+ T cell.
Single treatment (8 μM) led to almost completed loss of productive HIV infection in CD4+ T cells.

RT-PCR[1]

Cell Line: PBMCs of viremic HIV-infected individuals.
Concentration: 8 μM.
Incubation Time: 2 days.
Result: Suppresses HIV transcription ex vivo in PBMCs of viremic HIV-infected individuals.

Cell Cytotoxicity Assay[1]

Cell Line: J-Lat cells.
Concentration: 0-80 μM.
Incubation Time: 1 and 3 days.
Result: Did not cause significant cell death at concentrations below 40 μM.
Treatment of J-Lat cells with ZL0580 (10 μM) also did not cause significant cell death on days 2, 7, and 14 compared with NC in both PMA-activated and unstimulated cells.
Molecular Weight

532.53

Formula

C₂₅H₂₃F₃N₄O₄S

CAS No.

2377151-10-3

SMILES

O=C(NC1=CC=C(S(N2CCC[[email protected]]2C(NC3=CC=CC=C3)=O)(=O)=O)C=C1)NC4=CC=C(C(F)(F)F)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (469.46 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8778 mL 9.3891 mL 18.7783 mL
5 mM 0.3756 mL 1.8778 mL 3.7557 mL
10 mM 0.1878 mL 0.9389 mL 1.8778 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.91 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

ZL0580ZL 0580ZL-0580HIVEpigenetic Reader DomainHuman immunodeficiency viruslowtoxicityPBMCInhibitorinhibitorinhibit

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ZL0580
Cat. No.:
HY-126428
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