2. Anti-infection Epigenetics
  3. HIV Epigenetic Reader Domain
  4. ZL0580

ZL0580, a structurally close analog of ZL0590, induces epigenetic suppression of HIV via selectively binding to BD1 domain of BRD4. ZL0580 induces HIV suppression by inhibiting Tat transactivation and transcription elongation as well as by inducing repressive chromatin structure at the HIV promoter.

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ZL0580 Chemical Structure

ZL0580 Chemical Structure

CAS No. : 2377151-10-3

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

ZL0580 Related Antibodies

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Description

ZL0580, a structurally close analog of ZL0590, induces epigenetic suppression of HIV via selectively binding to BD1 domain of BRD4. ZL0580 induces HIV suppression by inhibiting Tat transactivation and transcription elongation as well as by inducing repressive chromatin structure at the HIV promoter[1][2][3].

IC50 & Target

BRD4 (BD1)

 

In Vitro

ZL0580 (8 μM, 2 days, PBMCs of viremic HIV-infected individuals) induces HIV transcriptional suppression with low toxicity[1].
ZL0580 treatment (10 μM) suppresses both PMA-stimulated and basal HIV transcription[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ZL0580 Related Antibodies

Cell Viability Assay[1]

Cell Line: HIV-infected human CD4+ T cells.
Concentration: 0-8 μM.
Incubation Time: 2 days.
Result: Suppress HIV in primary CD4+ T cell.
Single treatment (8 μM) led to almost completed loss of productive HIV infection in CD4+ T cells.

RT-PCR[1]

Cell Line: PBMCs of viremic HIV-infected individuals.
Concentration: 8 μM.
Incubation Time: 2 days.
Result: Suppresses HIV transcription ex vivo in PBMCs of viremic HIV-infected individuals.

Cell Cytotoxicity Assay[1]

Cell Line: J-Lat cells.
Concentration: 0-80 μM.
Incubation Time: 1 and 3 days.
Result: Did not cause significant cell death at concentrations below 40 μM.
Treatment of J-Lat cells with ZL0580 (10 μM) also did not cause significant cell death on days 2, 7, and 14 compared with NC in both PMA-activated and unstimulated cells.
Molecular Weight

532.53

Formula

C25H23F3N4O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(S(N2CCC[C@H]2C(NC3=CC=CC=C3)=O)(=O)=O)C=C1)NC4=CC=C(C(F)(F)F)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (469.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8778 mL 9.3891 mL 18.7783 mL
5 mM 0.3756 mL 1.8778 mL 3.7557 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8778 mL 9.3891 mL 18.7783 mL 46.9457 mL
5 mM 0.3756 mL 1.8778 mL 3.7557 mL 9.3891 mL
10 mM 0.1878 mL 0.9389 mL 1.8778 mL 4.6946 mL
15 mM 0.1252 mL 0.6259 mL 1.2519 mL 3.1297 mL
20 mM 0.0939 mL 0.4695 mL 0.9389 mL 2.3473 mL
25 mM 0.0751 mL 0.3756 mL 0.7511 mL 1.8778 mL
30 mM 0.0626 mL 0.3130 mL 0.6259 mL 1.5649 mL
40 mM 0.0469 mL 0.2347 mL 0.4695 mL 1.1736 mL
50 mM 0.0376 mL 0.1878 mL 0.3756 mL 0.9389 mL
60 mM 0.0313 mL 0.1565 mL 0.3130 mL 0.7824 mL
80 mM 0.0235 mL 0.1174 mL 0.2347 mL 0.5868 mL
100 mM 0.0188 mL 0.0939 mL 0.1878 mL 0.4695 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZL05802377151-10-3ZL 0580ZL-0580HIVEpigenetic Reader DomainHuman immunodeficiency viruslowtoxicityPBMCInhibitorinhibitorinhibit

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