α2,3-Sialyltransferase, Pasteurella multocida (P-1059)  (Synonyms: PmST1(M144D))

α2,3-Sialyltransferase, Pasteurella multocida (P-1059) (PmST1(M144D)), a sialyltransferase, can be isolated from Pasteurella multocida. alpha-2,3-Sialyltransferase (PmST1) can be converted into regioselective α2-6-sialyltransferase by saturation mutagenesis and regioselective screening^[1].

Product Information
This enzyme is used to connect sialic acid to the non-reducing end Gal in an α2,3 connection. This product is recombinantly expressed in E. coli, with a C-terminal His Tag, a molecular weight of 48.1 kDa, and a storage buffer component of 20 mM Tris-HCl, pH 8.0, and 10% glycerol.

Instructions
1. Add substrate and 5 mM MgCl₂ according to the reaction system. You can add appropriate buffer salts (such as Tris-HCl), adjust the pH to 8.5, and add appropriate amount of enzyme to react (according to the reaction requirements);
2. After mixing, incubate the sample at 37°C for 1-2h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

71124-51-1

Liquid

Colorless to light yellow

[a2,3-Sialyltransferase, Pasteurella multocida (P-1059)]

2.4.99.4

≥100 U/mL

One unit is defined as the amount of enzyme that transfer 1 μmol Neu5Ac from CMP-Neu5Ac to lacSph to form Neu5Acα2, 3Galβ1, 4Glc per min at 37℃.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. McArthur JB, et al. Converting Pasteurella multocidaα2-3-sialyltransferase 1 (PmST1) to a regioselective α2-6-sialyltransferase by saturation mutagenesis and regioselective screening. Org Biomol Chem. 2017 Feb 21;15(7):1700-1709.  [Content Brief]