α2,3-Sialyltransferase, Pasteurella multocida (P-1059)
Based on 1 Customer Validation
α2,3-Sialyltransferase, Pasteurella multocida (P-1059) (PmST1(M144D)), a sialyltransferase, can be isolated from Pasteurella multocida. alpha-2,3-Sialyltransferase (PmST1) can be converted into regioselective α2-6-sialyltransferase by saturation mutagenesis and regioselective screening.
For research use only. We do not sell to patients.
- CAS No.: 71124-51-1
- Molecular Weight:48.1 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Product Information
This enzyme is used to connect sialic acid to the non-reducing end Gal in an α2,3 connection. This product is recombinantly expressed in E. coli, with a C-terminal His Tag, a molecular weight of 48.1 kDa, and a storage buffer component of 20 mM Tris-HCl, pH 8.0, and 10% glycerol.
Instructions
1. Add substrate and 5 mM MgCl2 according to the reaction system. You can add appropriate buffer salts (such as Tris-HCl), adjust the pH to 8.5, and add appropriate amount of enzyme to react (according to the reaction requirements);
2. After mixing, incubate the sample at 37°C for 1-2h.
Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
2.4.99.4
Enzyme Activity
≥100 U/mL
Unit Definition
One unit is defined as the amount of enzyme that transfer 1 μmol Neu5Ac from CMP-Neu5Ac to lacSph to form Neu5Acα2, 3Galβ1, 4Glc per min at 37℃.
Technical Parameters
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Source
E. coli
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Tag
C-terminal His Tag
Chemical Information
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CAS No. 71124-51-1
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Appearance Liquid
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Molecular Weight 48.1 kDa
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Color Colorless to light yellow
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SMILES
[a2,3-Sialyltransferase, Pasteurella multocida (P-1059)]
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Synonyms
PmST1(M144D)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
α2,3-Sialyltransferase, Pasteurella multocida (P-1059) Related Classifications
HY-E70056 Related Classifications
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