2. Metabolic Enzyme/Protease
  3. Drug Metabolite
  4. 1,1,3-Tribromoacetone

1,1,3-Tribromoacetone is an impurity of Methotrexate (HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis.

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1,1,3-Tribromoacetone Chemical Structure

1,1,3-Tribromoacetone Chemical Structure

CAS No. : 3475-39-6

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250 mg USD 90 In-stock
500 mg USD 130 In-stock
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1,1,3-Tribromoacetone Related Antibodies

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Description

1,1,3-Tribromoacetone is an impurity of Methotrexate (HY-14519)[1]. Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis[1].

In Vivo

Methotrexate (Amethopterin) reduces thymus and spleen indices of mice. Methotrexate markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate exposure on indices of thymus and spleens in mice[2].
Methotrexate (MTX) (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund's complete adjuvant-induced arthritis. The combination of Methotrexate (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

294.77

Formula

C3H3Br3O
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=C(CBr)C(Br)Br
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (339.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3925 mL 16.9624 mL 33.9248 mL
5 mM 0.6785 mL 3.3925 mL 6.7850 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3925 mL 16.9624 mL 33.9248 mL 84.8119 mL
5 mM 0.6785 mL 3.3925 mL 6.7850 mL 16.9624 mL
10 mM 0.3392 mL 1.6962 mL 3.3925 mL 8.4812 mL
15 mM 0.2262 mL 1.1308 mL 2.2617 mL 5.6541 mL
20 mM 0.1696 mL 0.8481 mL 1.6962 mL 4.2406 mL
25 mM 0.1357 mL 0.6785 mL 1.3570 mL 3.3925 mL
30 mM 0.1131 mL 0.5654 mL 1.1308 mL 2.8271 mL
40 mM 0.0848 mL 0.4241 mL 0.8481 mL 2.1203 mL
50 mM 0.0678 mL 0.3392 mL 0.6785 mL 1.6962 mL
60 mM 0.0565 mL 0.2827 mL 0.5654 mL 1.4135 mL
80 mM 0.0424 mL 0.2120 mL 0.4241 mL 1.0601 mL
100 mM 0.0339 mL 0.1696 mL 0.3392 mL 0.8481 mL
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1,1,3-Tribromoacetone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1,1,3-Tribromoacetone3475-39-6Drug MetaboliteImpurityMethotrexateInhibitorinhibitorinhibit

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