16S rRNA Pseudouridine516 synthase

16S rRNA Pseudouridine516 synthase (EC 5.4.99.19) is specific for uridine516 in 16S rRNA. In vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins.

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