2'-Nitroflavone 

2'-Nitroflavone is a PARP1 inhibitor. 2'-Nitroflavone inhibits the proliferation, induces cell cycle arrest and apoptosis of triple-negative breast cancer cells. 2'-Nitroflavone also inhibits the migration of triple-negative breast cancer cells and endothelial cells. 2'-Nitroflavone exhibits antitumor activity and can be used in the research of tumors such as triple-negative breast cancer^[1].

2'-Nitroflavone (1.25-40 μM; 0-96 h) inhibits the viability of three triple-negative breast cancer (TNBC) cell lines, MDA-MB-231, BT-549 and MDA-MB-468, with IC₅₀ values of 4.0 μM, 9.2 μM and 6.5 μM after 72 h of treatment, respectively and has IC₅₀ against EA.hy926 endothelial cells of 14.5 μM at 96 h^[1].
2'-Nitroflavone (0-25 μM; 72-144 h) inhibits colony formation and migration of triple-negative breast cancer cells, and induces cell cycle arrest and apoptosis^[1].
2'-Nitroflavone (1-25 μM; 72 h) reduces the protein level of α-tubulin in MDA-MB-231 and BT-549 cells, disrupts the arrangement of α-tubulin in cells, and induces the formation of multipolar mitotic spindles^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2'-Nitroflavone (20 mg/kg; i.p.; 5 times per week for 2 consecutive weeks) exhibits anti-tumor activity in the 4T1 triple-negative breast cancer mouse model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

267.24

C₁₅H₉NO₄

53277-26-2

O=C1C2=CC=CC=C2OC(C3=C([N+]([O-])=O)C=CC=C3)=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Vachetta VS, et al. 2'-Nitroflavone targets PARP1 and alters cytoskeleton to inhibit triple-negative breast cancer progression. Sci Rep. 2026;16(1):4164. Published 2026 Jan 3.  [Content Brief]