27-O-Acetyl-withaferin A

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27-O-Acetyl-withaferin A is found in Withania aristata.

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27-O-Acetyl-withaferin A Chemical Structure

CAS No. : 1214886-35-7

Size	Stock
5 mg	Get quote	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31

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Biological Activity
Purity & Documentation
References
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Description

27-O-Acetyl-withaferin A is found in Withania aristata^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	4.4 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against human A549 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 22705001]
A549	IC₅₀	6.7 μM Compound: 2	Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
A549	IC₅₀	6.7 μM Compound: 2	Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
A549	IC₅₀	6.7 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
DU-145	IC₅₀	0.8 μM Compound: 2	Cytotoxicity against human hormone nonresponsive DU-145 cells assessed as reduction in cell proliferation by MTT assay Cytotoxicity against human hormone nonresponsive DU-145 cells assessed as reduction in cell proliferation by MTT assay	[PMID: 38414352]
HFF	IC₅₀	1.9 μM Compound: 2	Cytotoxicity against human HFF cells assessed as reduction in cell proliferation by MTT assay Cytotoxicity against human HFF cells assessed as reduction in cell proliferation by MTT assay	[PMID: 38414352]
HeLa	IC₅₀	10.2 μM Compound: 2	Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
HeLa	IC₅₀	4.7 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against human HeLa cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 22705001]
HeLa	IC₅₀	7.7 μM Compound: 2	Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
HeLa	IC₅₀	7.7 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
LNCaP	IC₅₀	1.9 μM Compound: 2	Cytotoxicity against human LNCaP cells assessed as reduction in cell proliferation by MTT assay Cytotoxicity against human LNCaP cells assessed as reduction in cell proliferation by MTT assay	[PMID: 38414352]
MCF7	IC₅₀	2 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against human MCF7 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 22705001]
MCF7	IC₅₀	4.6 μM Compound: 2	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
MCF7	IC₅₀	5.3 μM Compound: 2	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
MCF7	IC₅₀	5.3 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
MM1.S	IC₅₀	114 nM Compound: 6	Antiproliferative activity against human MM1.S cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay Antiproliferative activity against human MM1.S cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay	[PMID: 34445874]
NCI-H929	IC₅₀	0.25 μM Compound: 15	Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay	[PMID: 24625088]
PC-3	IC₅₀	0.3 μM Compound: 2	Cytotoxicity against human hormone nonresponsive PC-3 cells assessed as reduction in cell proliferation by MTT assay Cytotoxicity against human hormone nonresponsive PC-3 cells assessed as reduction in cell proliferation by MTT assay	[PMID: 38414352]
RPMI-8226	IC₅₀	1.51 μM Compound: 6	Antiproliferative activity against human RPMI-8226 cells cultured as 3D-spheroids assessed as reduction in cell viability after 48 hrs by Celltiter-Glo assay Antiproliferative activity against human RPMI-8226 cells cultured as 3D-spheroids assessed as reduction in cell viability after 48 hrs by Celltiter-Glo assay	[PMID: 34445874]
RPMI-8226	IC₅₀	212 nM Compound: 6	Antiproliferative activity against human RPMI 8226 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay Antiproliferative activity against human RPMI 8226 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay	[PMID: 34445874]
Vero	IC₅₀	11.4 μM Compound: 2	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
Vero	IC₅₀	11.4 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
Vero	IC₅₀	5.9 μM Compound: 2	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
Vero	IC₅₀	8.5 μM Compound: 2, 27-O-acetyl-withaferin A	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 22705001]

Molecular Weight

512.63

Formula

C₃₀H₄₀O₇

CAS No.

1214886-35-7

SMILES

C[C@]12[C@@]3([C@H](C=CC2=O)O)[C@](C[C@]4([H])[C@]1([H])CC[C@]5([C@@]4([H])CC[C@]5([H])[C@@H]([C@@]6([H])CC(C)=C(C(O6)=O)COC(C)=O)C)C)([H])O3

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Llanos GG, Araujo LM, Jiménez IA, Moujir LM, Bazzocchi IL. Withaferin A-related steroids from Withania aristata exhibit potent antiproliferative activity by inducing apoptosis in human tumor cells. Eur J Med Chem. 2012;54:499-511. [Content Brief]