1. (5)6-Carboxytetramethylrhodamine

(5)6-Carboxytetramethylrhodamine 

Cat. No.: HY-D0934 Purity: 98.29%
COA Handling Instructions

(5)6-Carboxytetramethylrhodamine contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm.

For research use only. We do not sell to patients.

(5)6-Carboxytetramethylrhodamine Chemical Structure

(5)6-Carboxytetramethylrhodamine Chemical Structure

CAS No. : 117557-83-2

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10 mg USD 80 In-stock
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Description

(5)6-Carboxytetramethylrhodamine contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm[1].

Molecular Weight

431.46

Formula

C25H23N2O5+

CAS No.
Appearance

Solid

Color

Gray to dark gray

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(C(O)=O)C=C4C(O)=O)=C2C=C1)C.CN(C5=CC6=[O+]C7=C(C=CC(N(C)C)=C7)C(C8=CC(C(O)=O)=CC=C8C(O)=O)=C6C=C5)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (46.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Methanol : 5 mg/mL (11.59 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3177 mL 11.5886 mL 23.1771 mL
5 mM 0.4635 mL 2.3177 mL 4.6354 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Methanol / DMSO 1 mM 2.3177 mL 11.5886 mL 23.1771 mL 57.9428 mL
5 mM 0.4635 mL 2.3177 mL 4.6354 mL 11.5886 mL
10 mM 0.2318 mL 1.1589 mL 2.3177 mL 5.7943 mL
DMSO 15 mM 0.1545 mL 0.7726 mL 1.5451 mL 3.8629 mL
20 mM 0.1159 mL 0.5794 mL 1.1589 mL 2.8971 mL
25 mM 0.0927 mL 0.4635 mL 0.9271 mL 2.3177 mL
30 mM 0.0773 mL 0.3863 mL 0.7726 mL 1.9314 mL
40 mM 0.0579 mL 0.2897 mL 0.5794 mL 1.4486 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
(5)6-Carboxytetramethylrhodamine
Cat. No.:
HY-D0934
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