Cat. No.: HY-W010342

6-Aminonicotinamide

22 Cited Publications

Customer Review

Based on 22 publication(s) in Google Scholar

6-Aminonicotinamide, a potent antimetabolite of nicotinamide, is competitive NADP⁺-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) inhibitor (K_i=0.46 μM). 6-Aminonicotinamide resultis ATP depletion and synergizes with DNA-crosslinking chemotherapy agents, such as Cisplatin (HY-17394), in killing cancer cells.

For research use only. We do not sell to patients.

Purity: 99.95%
CAS No.: 329-89-5
Formula: C₆H₇N₃O
Molecular Weight:137.14

Storage:

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

6-Aminonicotinamide Chemical Structure

CAS No.: 329-89-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All NADPH Oxidase Isoforms :

In Vitro

6-Aminonicotinamide (100 nM; 7 days) causes a significant decrease in the human AR+, hormone-sensitive prostate cancer cell lines LNCaP and LAPC4, as well as the CRPC-derivative C4-2 and 22Rv1 cell models. 6-Aminonicotinamide (100 nM ± 10 nM R1881 as indicated for 3 days) increases both basal- and R1881-mediated ROS levels, suggesting 6-Aminonicotinamide is blocking the cells' antioxidant defense. 6-Aminonicotinamide also increases ROS levels in C4-2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

6-Aminonicotinamide (20 mg/kg; i.p.; thress times; days 1, 10 or 11, and 21) alone induces a small but significant tumor growth delay (4.3+/-0.8 days). Treatment with 6-Aminonicotinamide followed by radiation induces a tumor growth delay of 57.0+/-3.8 days in CD8F1 breast tumor model^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 329-89-5
Appearance Solid
Molecular Weight 137.14
Formula C₆H₇N₃O
Color White to off-white
SMILES

NC1=CC=C(C(N)=O)C=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Publications (22)

All In Vivo Efficacy StudyCell Proliferation/Viability AssayELISAIFFlow Cytometry

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Journal Impact Factor

Nat Biomed Eng

A live bacteria enzyme assay for identification of human disease mutations and drug screening. [Abstract]

2025 Sep;9(9):1547-1556. PMID: 40307426

6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Nat Biomed Eng. 2025 Sep;9(9):1547-1556. [Abstract]

Growth rates of humanized E. coli in LEICA with different compounds. 6-Aminonicotinamide (6AN) (100 μM) completely halted the growth of humanized E. coli for G6PD in LEICA.
Immunity

Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosis. [Abstract]

2026 Feb 24:S1074-7613(26)00030-0. PMID: 41742421
Cell Death Differ

A neutrophil-intrinsic CKLF1-PKM2 axis drives glycolytic flux for de novo DAG synthesis and pro-inflammatory ROS production. [Abstract]

2026 May 12. PMID: 42120940
Redox Biol

Glucose-6-phosphate dehydrogenase alleviates epileptic seizures by repressing reactive oxygen species production to promote signal transducer and activator of transcription 1-mediated N-methyl-d-aspartic acid receptors inhibition. [Abstract]

2024 Aug:74:103236. PMID: 38875958
Cancer Lett

GPI inactivation mediates pentose phosphate pathway flux switch-on inducing temozolomide resistance in glioma stem cell. [Abstract]

2026 May 1:645:218384. PMID: 41763452
Cell Death Dis

Post-irradiation dietary restriction impairs hematopoiesis via inhibition of the pentose phosphate pathway in hematopoietic stem and progenitor cells. [Abstract]

2026 Jan 7;17(1):8. PMID: 41500989

6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]

Experimental scheme. Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays and fed with an AL diet. Mice were then injected intraperitoneally with 10 mg/kg of 6-Aminonicotinamide (6-AN) or saline daily for 7 days post-irradiation, and were continued on an AL diet until 1 month post-irradiation. G6PD enzyme activity (B) and NADPH content (C) in BM cells of AL mice 1 month post-irradiation, analyzed using ELISA.

6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]

Representative images of γH2AX and 53BP1 colocalization in BM cells of AL mice 1 month post-irradiation, determined by immunofluorescent staining. Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays and fed with an AL diet. Mice were then injected intraperitoneally with 10 mg/kg of 6-Aminonicotinamide (6-AN) or saline daily for 7 days post-irradiation, and were continued on an AL diet until 1 month post-irradiation.

6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]

Representative FACS Plots of HSCs (Left) and CMPs/GMPs/MEPs (Right) of AL mice 1 month post-irradiation. .Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays and fed with an AL diet. Mice were then injected intraperitoneally with 10 mg/kg of 6-Aminonicotinamide (6-AN) or saline daily for 7 days post-irradiation, and were continued on an AL diet until 1 month post-irradiation.
Phytomedicine

Gambogic acid exhibits promising anticancer activity by inhibiting the pentose phosphate pathway in lung cancer mouse model. [Abstract]

2024 Jul:129:155657. PMID: 38692076
Cell Rep

SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. [Abstract]

2026 Apr 25;45(5):117297. PMID: 42035418
PLoS Pathog

Nicotinamide metabolism is essential for Hepatitis C Virus replication and the production of infectious Lipo-Viro-Particles. [Abstract]

2026 Apr 22;22(4):e1014165. PMID: 42018568
PLoS Pathog

White spot syndrome virus IE1 protein hijacks the host pentose phosphate pathway to fuel viral replication. [Abstract]

2026 Jan 27;22(1):e1013913. PMID: 41592137
Int J Mol Sci

PIP5Kγ Mediates PI(4,5)P2/Merlin/LATS1 Signaling Activation and Interplays with Hsc70 in Hippo-YAP Pathway Regulation. [Abstract]

2023 Sep 30;24(19):14786. PMID: 37834234
J Nutr Biochem

F6P/G6P-mediated ChREBP activation promotes the insulin resistance-driven hepatic lipid deposition in zebrafish. [Abstract]

2023 Dec:122:109452. PMID: 37748621
J Cereb Blood Flow Metab

G6PD deficiency is implicated in Circle of Willis variants in stroke patients and impairs brain vasculature in zebrafish. [Abstract]

2025 Sep 25:271678X251377678. PMID: 40995861
J Biol Chem

G6PD facilitates axon regeneration via clathrin-mediated endocytosis. [Abstract]

2026 Apr;302(4):111345. PMID: 41791708
J Photochem Photobiol B

14-3-3σ alleviates UVB-induced epidermal oxidative stress through the pentose phosphate pathway promotion. [Abstract]

2026 Jun:279:113441. PMID: 42001822
Eur J Immunol

Hyperinflammation by Human Macrophages Induced by SARS-CoV-2 Anti-Spike IgG Is Dependent on Glucose and Fatty Acid Metabolism. [Abstract]

2025 Dec;55(12):e70087. PMID: 41416928
Eur J Immunol

Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages. [Abstract]

2025 Oct;55(10):e70068. PMID: 41054024

6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Eur J Immunol. 2025 Oct;55(10):e70068. [Abstract]

Alveolar-like macrophages co-stimulated with Poly(I:C) and anti-spike IgA were tested for their dependency on glycolysis, the pentose phosphate pathway, and fatty acid metabolism. Cells preincubated with metabolic inhibitors (6-Aminonicotinamide; 6-AN; 0.1 mM; 24 h) were stimulated and analyzed for their production of IL-6.
Metabolites

Lipidomics and Transcriptomics Differ Liposarcoma Differentiation Characteristics That Can Be Altered by Pentose Phosphate Pathway Intervention. [Abstract]

2022 Dec 7;12(12):1227. PMID: 36557266

6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Metabolites. 2022 Dec 7;12(12):1227. [Abstract]

6-Aminonicotinamide (6-AN; 1 μM; 0-4 days) significantly promotes the proliferation of LPS853 cells.
bioRxiv

ATGL-catalyzed lipid catabolism promotes DNA repair. [Abstract]

2026 Apr 4:2026.04.03.716381. PMID: 41959418
bioRxiv

2025 Oct 14.
University of Kentucky

2025.
Research Square Print

2023 Feb 14.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (729.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 11.11 mg/mL (81.01 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.2917 mL

36.4586 mL

72.9171 mL

5 mM

1.4583 mL

7.2917 mL

14.5834 mL

10 mM

0.7292 mL

3.6459 mL

7.2917 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 4 mg/mL (29.17 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cheng J, et al. TRIM21 and PHLDA3 negatively regulate the crosstalk between the PI3K/AKT pathway and PPP metabolism. Nat Commun. 2020;11(1):1880. Published 2020 Apr 20. [Content Brief]

[2]. Almugadam SH, et al. Influence of 6-aminonicotinamide (6AN) on Leishmania promastigotes evaluated by metabolomics: Beyond the pentose phosphate pathway. Chem Biol Interact. 2018;294:167-177. [Content Brief]

[3]. Tsouko E, et al. Regulation of the pentose phosphate pathway by an androgen receptor-mTOR-mediated mechanism and its role in prostate cancer cell growth. Oncogenesis. 2014;3(5):e103. Published 2014 May 26. [Content Brief]

[4]. Koutcher JA, et al. Effect of 6-aminonicotinamide on the pentose phosphate pathway: 31P NMR and tumor growth delay studies. Magn Reson Med. 1996;36(6):887-892. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	7.2917 mL	36.4586 mL	72.9171 mL	182.2928 mL
	5 mM	1.4583 mL	7.2917 mL	14.5834 mL	36.4586 mL
	10 mM	0.7292 mL	3.6459 mL	7.2917 mL	18.2293 mL
	15 mM	0.4861 mL	2.4306 mL	4.8611 mL	12.1529 mL
	20 mM	0.3646 mL	1.8229 mL	3.6459 mL	9.1146 mL
	25 mM	0.2917 mL	1.4583 mL	2.9167 mL	7.2917 mL
	30 mM	0.2431 mL	1.2153 mL	2.4306 mL	6.0764 mL
	40 mM	0.1823 mL	0.9115 mL	1.8229 mL	4.5573 mL
	50 mM	0.1458 mL	0.7292 mL	1.4583 mL	3.6459 mL
	60 mM	0.1215 mL	0.6076 mL	1.2153 mL	3.0382 mL
	80 mM	0.0911 mL	0.4557 mL	0.9115 mL	2.2787 mL
DMSO	100 mM	0.0729 mL	0.3646 mL	0.7292 mL	1.8229 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

6-Aminonicotinamide

22 Cited Publications

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6-Aminonicotinamide

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Glycolysis Compound Library

Glutamine Metabolism Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

MCE Kinase Hinge Binder Fragment Library

Publications Citing Use of MedChemExpress (MCE) 6-Aminonicotinamide

All NADPH Oxidase Isoforms

Biological Activity

Chemical Information

Publications (22)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

6-Aminonicotinamide

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Glycolysis Compound Library

Glutamine Metabolism Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

MCE Kinase Hinge Binder Fragment Library

Publications Citing Use of MedChemExpress (MCE) 6-Aminonicotinamide

All NADPH Oxidase Isoforms

Biological Activity

Chemical Information

Publications (22)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

6-Aminonicotinamide Related Classifications

HY-W010342 Related Classifications