Galloflavin
Based on 17 publication(s) in Google Scholar
Galloflavin is a potent lactate dehydrogenase (LDH) inhibitor. The calculated Kis for pyruvate are 5.46 μM (LDH-A) and 15.06 μM (LDH-B). Galloflavin hinders the proliferation of cancer cells by blocking glycolysis and ATP production.
For research use only. We do not sell to patients.
- Purity: 99.36%
- CAS No.: 568-80-9
- Formula: C12H6O8
- Molecular Weight:278.17
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Glycolysis Compound Library
HY-L058
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Anti-Cancer Metabolism Compound Library
HY-L083
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Glucose Metabolism Compound Library
HY-L092
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Lactic Acid Metabolic Compound Library
HY-L204
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High-Throughput Bioactive Compound Library
HY-L205
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Lactylation Compound Library
HY-L249
Publications Citing Use of MedChemExpress (MCE) Galloflavin
More- Cell Res. 2023 Sep;33(9):679-698. [Abstract]
- Drug Resist Updat. 2024 Mar:73:101060. [Abstract]
- Mol Cell. 2024 Feb 15;84(4):744-759.e6. [Abstract]
- Redox Biol. 2024 May:71:103112. [Abstract]
- J Immunother Cancer. 2025 Dec 3;13(12):e013733. [Abstract]
- Cell Commun Signal. 2025 Feb 19;23(1):99. [Abstract]
- Cell Rep. 2024 Dec 12;43(12):115065. [Abstract]
- Sci Signal. 2026 Mar 3;19(927):eadz6443. [Abstract]
- J Dent. 2026 Jun:169:106664. [Abstract]
- Commun Biol. 2025 Nov 26;8(1):1761. [Abstract]
- Commun Biol. 2025 Jul 8;8(1):1020. [Abstract]
- Int Immunopharmacol. 2025 Mar 26:150:114265. [Abstract]
- J Immunol. 2025 Nov 6:vkaf218. [Abstract]
- Toxicol Appl Pharmacol. 2025 Aug 10:504:117508. [Abstract]
- SSRN. 2026 Apr 11.
- SSRN. 2025 Dec 2.
- bioRxiv. 2025 Jul 26:2025.07.22.666143. [Abstract]
Customer Validation & Images
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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Apoptosis Analysis
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Cell Imaging/Staining
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IP
Biological Activity
Galloflavin effectively inhibits cell growth in endometrial cancer cell lines and primary cultures of human endometrial cancer through its involvement in multiple signaling pathways that regulate metabolism, cell cycle, apoptosis, cell stress and metastasis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 568-80-9
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Appearance Solid
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Molecular Weight 278.17
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Formula C12H6O8
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Color Yellow to brown
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SMILES
O=C(OC(C=C1O)=C2OC1=O)C3=C2C(O)=C(O)C(O)=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (17)
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Journal Impact Factor
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Most Recent
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Cell Res
α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure. [Abstract]2023 Sep;33(9):679-698. PMID: 37443257
Galloflavin purchased from MedChemExpress. Usage Cited in: Cell Res. 2023 Sep;33(9):679-698. [Abstract]
H9c2 cells stimulated with or without Galloflavin (LDHA inhibitor; 10 μM; 24 h) were lysed and immunoprecipitated using anti-α-MHC antibody or control IgG, followed by detection of αMHC K1897 Lactyl Lysine.
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Drug Resist Updat
Polyphenol nanocomplex modulates lactate metabolic reprogramming and elicits immune responses to enhance cancer therapeutic effect. [Abstract]2024 Mar:73:101060. PMID: 38309140
Galloflavin purchased from MedChemExpress. Usage Cited in: Drug Resist Updat. 2024 Mar:73:101060. [Abstract]
Inhibition of extracellular lactate secretion after different treatments (top). The concentration of pyruvate after various treatments (bottom). PBS, Galloflavin (GF;8 μM), and PCGF (containing GF 8 μM) were used to coculture with 4T1 cells. After incubation for 48 h, the tumor cell supernatant was collected for lactate and pyruvate measurement.
Galloflavin purchased from MedChemExpress. Usage Cited in: Drug Resist Updat. 2024 Mar:73:101060. [Abstract]
Cell viability after various treatments. The MTT assay was conducted after various treatments (PBS, CA 10 µM, Galloflavin (GF) 8 µM, PCGF (equivalent to CA 10 µM, GF 8 µM), and PCGF+Laser) for 48 h in 4T1 cells.
Galloflavin purchased from MedChemExpress. Usage Cited in: Drug Resist Updat. 2024 Mar:73:101060. [Abstract]
Characteristic flow cytometry profiles depicting 4T1 cell apoptosis post different treatment interventions. PBS, CA 10 µM, Galloflavin (GF) 8 µM, PCGF (equivalent to CA 10 µM, GF 8 µM), and PCGF+Laser) for 24 h.
Galloflavin purchased from MedChemExpress. Usage Cited in: Drug Resist Updat. 2024 Mar:73:101060. [Abstract]
Results of the live/dead co-staining assay after different treatments (top). Levels of DNA double-strand breaks in 4T1 cells following treatments with PBS, CA, Galloflavin (GF), PCGF, and PCGF+Laser (bottom). PBS, CA 10 µM, Galloflavin (GF) 8 µM, PCGF (equivalent to CA 10 µM, GF 8 µM), and PCGF+Laser) for 24 h.
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Mol Cell
Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation. [Abstract]2024 Feb 15;84(4):744-759.e6. PMID: 38266638 -
Redox Biol
Enteric coronavirus PDCoV evokes a non-Warburg effect by hijacking pyruvic acid as a metabolic hub. [Abstract]2024 May:71:103112. PMID: 38461791 -
J Immunother Cancer
2025 Dec 3;13(12):e013733. PMID: 41339107 -
Cell Commun Signal
Intrinsic STING of CD8 + T cells regulates self-metabolic reprogramming and memory to exert anti-tumor effects. [Abstract]2025 Feb 19;23(1):99. PMID: 39972350 -
Cell Rep
Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy. [Abstract]2024 Dec 12;43(12):115065. PMID: 39671292 -
Sci Signal
Glucose metabolism sustains aberrant STAT3 signaling in colorectal cancer through glycosylated local signaling factors. [Abstract]2026 Mar 3;19(927):eadz6443. PMID: 41774818 -
J Dent
2026 Jun:169:106664. PMID: 41921651 -
Commun Biol
Nicotinamide mononucleotide treatment improves spermatogenesis in obese mice by reducing lysine acetylation of lactate dehydrogenase C. [Abstract]2025 Nov 26;8(1):1761. PMID: 41298813 -
Commun Biol
CAF-derived GLUT1 and its role in modulating ovarian cancer progression: a multi-dimensional analysis of the tumor microenvironment. [Abstract]2025 Jul 8;8(1):1020. PMID: 40629043 -
Int Immunopharmacol
Galloflavin mitigates acute kidney injury by suppressing LDHA-dependent macrophage glycolysis. [Abstract]2025 Mar 26:150:114265. PMID: 39955920 -
J Immunol
The involvement of CLec-HTM/IgR-Syk-BCL10-mTOR/ERK signaling pathway in immune priming of oysters. [Abstract]2025 Nov 6:vkaf218. PMID: 41206721 -
Toxicol Appl Pharmacol
Myocardial ischemia/reperfusion-induced glycolysis enhances damage through TRPM7 histone lactylation. [Abstract]2025 Aug 10:504:117508. PMID: 40796076 -
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bioRxiv
Glucose Metabolism Sustains Aberrant STAT3 Signaling in Colorectal Cancer via Glycosylated Paracrine Factors. [Abstract]2025 Jul 26:2025.07.22.666143. PMID: 40777356
Solvent & Solubility
DMSO : 5 mg/mL (17.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (1.80 mM); Clear solution
This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.5 mg/mL (1.80 mM); Clear solution
This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (89.87 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Manerba M, Vettraino M, Fiume L, et al. Galloflavin (CAS 568-80-9): a novel inhibitor of lactate dehydrogenase. ChemMedChem. 2012;7(2):311-317. [Content Brief]
[2]. Farabegoli F, et al. Galloflavin, a new lactate dehydrogenase inhibitor, induces the death of human breast cancer cells with different glycolytic attitude by affecting distinct signaling pathways. Eur J Pharm Sci. 2012;47(4):729-738. [Content Brief]
[3]. Han X, et al. Evaluation of the anti-tumor effects of lactate dehydrogenase inhibitor galloflavin in endometrial cancer cells. J Hematol Oncol. 2015;8:2. Published 2015 Jan 29. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5949 mL | 17.9745 mL | 35.9490 mL | 89.8725 mL |
| 5 mM | 0.7190 mL | 3.5949 mL | 7.1898 mL | 17.9745 mL | |
| 10 mM | 0.3595 mL | 1.7974 mL | 3.5949 mL | 8.9872 mL | |
| 15 mM | 0.2397 mL | 1.1983 mL | 2.3966 mL | 5.9915 mL |
Galloflavin Related Classifications
HY-W040118 Related Classifications
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