7-Methoxycoumarin-3-carboxylic acid
Based on 1 Customer Validation
7-Methoxycoumarin-3-carboxylic acid is a fluorescent dye with an excitation peak at 355 nm and an emission peak at 405 nm. 7-Methoxycoumarin-3-carboxylic acid can be used to label peptide.
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- Pureza: 97.0%
- No. CAS: 20300-59-8
- Fòrmula: C11H8O5
- Peso molecular:220.18
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
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Cell Line
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Type | Value | Description | References |
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| Oocyte | IC50 |
168 μM
Compound: 7-OMe-Coum-COOH
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Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique
Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique
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[PMID: 23682762] |
| SiHa | EC50 |
9.8 μM
Compound: 1
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Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
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[PMID: 24095010] |
| SiHa | IC50 |
2.3 μM
Compound: 1
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Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
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[PMID: 24095010] |
7-Methoxycoumarin-3-carboxylic acid (compound 5) (100 μM; 72 h) shows non-toxic for K562, Daudi, RPMI-8226, and Jurkat cells with IC50s of >100, >100, >100, >100 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
450
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Chemical Information
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No. CAS 20300-59-8
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Appearance Solid
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Peso molecular 220.18
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Fòrmula C11H8O5
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Color White to off-white
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SMILES
O=C(C1=CC2=CC=C(OC)C=C2OC1=O)O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
DMSO : 8 mg/mL (36.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Wiemer AJ, et al. Evaluation of a 7-Methoxycoumarin-3-carboxylic Acid Ester Derivative as a Fluorescent, Cell-Cleavable, Phosphonate Protecting Group. Chembiochem. 2016 Jan 1;17(1):52-5. [Content Brief]
[2]. Mohamed-Anis Alouini, et al. Interaction of fluorescently labeled triethyleneglycol and peptide derivatives with β-cyclodextrin. Chemphyschem. 2014 Feb 24;15(3):444-57. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.5417 mL | 22.7087 mL | 45.4174 mL | 113.5435 mL |
| 5 mM | 0.9083 mL | 4.5417 mL | 9.0835 mL | 22.7087 mL | |
| 10 mM | 0.4542 mL | 2.2709 mL | 4.5417 mL | 11.3543 mL | |
| 15 mM | 0.3028 mL | 1.5139 mL | 3.0278 mL | 7.5696 mL | |
| 20 mM | 0.2271 mL | 1.1354 mL | 2.2709 mL | 5.6772 mL | |
| 25 mM | 0.1817 mL | 0.9083 mL | 1.8167 mL | 4.5417 mL | |
| 30 mM | 0.1514 mL | 0.7570 mL | 1.5139 mL | 3.7848 mL |
7-Methoxycoumarin-3-carboxylic acid Related Classifications
HY-W039519 Related Classifications
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