8MDP
Based on 1 Customer Validation
8MDP is a potent equilibrative nucleoside transporter 1 (ENT1) inhibitor with an IC50 of 0.43 nM. 8MDP inhibits hENT1 and hENT2 uptake of [H3] uridine by K562 cells and K15NTD cells.
For research use only. We do not sell to patients.
- Purity : 97.9%
- CAS No.: 29491-75-6
- Formula: C28H48N8O4
- Molecular Weight:560.73
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
Equilibrative Nucleoside Transporter 1 (ENT1)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| K562 | IC50 |
8.67 nM
Compound: 13
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Inhibition of ENT1 in human K562 cells by flow cytometric assay
Inhibition of ENT1 in human K562 cells by flow cytometric assay
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[PMID: 17636949] |
Chemical Information
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CAS No. 29491-75-6
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Appearance Solid
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Molecular Weight 560.73
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Formula C28H48N8O4
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Color Light yellow to green yellow
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SMILES
OCCN(CCO)C1=NC(N2CCCCCCC2)=C(N=C(N(CCO)CCO)N=C3N4CCCCCCC4)C3=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Lin W, et al. Design, synthesis, and evaluation of 2-diethanolamino-4,8-diheptamethyleneimino-2-(N-aminoethyl-N-ethanolamino)-6-(N,N-diethanolamino)pyrimido[5,4-d]pyrimidine-fluorescein conjugate (8MDP-fluor), as a novel equilibrative nucleoside transporter probe. Bioconjug Chem. 2011 Jun 15;22(6):1221-7. [Content Brief]
[2]. Lin W, et al. Synthesis, flow cytometric evaluation, and identification of highly potent dipyridamole analogues as equilibrative nucleoside transporter 1 inhibitors. J Med Chem. 2007 Aug 9;50(16):3906-20. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
8MDP Related Classifications
HY-110104 Related Classifications
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