1. JAK/STAT Signaling
    Stem Cell/Wnt
  2. STAT

Alantolactone (Synonyms: (+)-Alantolactone; Alant camphor; Inula camphor)

Cat. No.: HY-N0038 Purity: 99.94%
Handling Instructions

Alantolactone is a selective STAT3 inhibitor, with potent anticancer activity.

For research use only. We do not sell to patients.

Alantolactone Chemical Structure

Alantolactone Chemical Structure

CAS No. : 546-43-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 96 In-stock
Estimated Time of Arrival: December 31
25 mg USD 192 In-stock
Estimated Time of Arrival: December 31
50 mg USD 336 In-stock
Estimated Time of Arrival: December 31
100 mg USD 540 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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    Alantolactone purchased from MCE. Usage Cited in: Stem Cells. 2019 Feb;37(2):190-201.

    Western blot images show Smad2/3 phosphorylation is lower than baseline in the sh-GPM6B cells and higher than baseline in the GPM6B-sgRNA cells. The phosphorylation of Smad2/3 in the sh-GPM6B TGF-β1 group is elevated by Alantolactone and the phosphorylation is reduced by treatment with SB431542.

    Alantolactone purchased from MCE. Usage Cited in: Stem Cells. 2019 Feb;37(2):190-201.

    Western blot analyses show the repression of SMC specific markers in the sh-GPM6B TGF-β1 group are abolished by Alantolactone in the sh-GPM6B and the enrichment of SMC specific markers in the GPM6B-sgRNA cells is cancelled by treatment with SB431542.

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    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Alantolactone is a selective STAT3 inhibitor, with potent anticancer activity.

    IC50 & Target

    STAT3

     

    In Vitro

    Alantolactone induces apoptosis in HepG2 cells in a dose-dependent manner. This Alantolactone-induced apoptosis is found to be associated with GSH depletion, inhibition of STAT3 activation, ROS generation, mitochondrial transmembrane potential dissipation, and increased Bax/Bcl-2 ratio and caspase-3 activation[1]. Alantolactone decreases STAT3 translocation to the nucleus, its DNA-binding, and STAT3 target gene expression. Alantolactone significantly inhibits STAT3 activation with a marginal effect on MAPKs and on NF-κB transcription; however, this effect is not mediated by inhibiting STAT3 upstream kinases[2].

    In Vivo

    It is found that the average tumor volume in the Alantolactone-treated mice is approximately 2.17-fold lower compared with that in the control mice. However the administration of Alantolactone does not affect the overall bodyweight during the experimental period, suggesting no apparent toxicity. Additionally, the average tumor weight is significantly lower in the Alantolactone-treated mice compared with the control mice. What’s more, the administration of Alantolactone results in a significant decrease in p-STAT3 and cyclin D1 expression in the tumor tissues[2].

    Solvent & Solubility
    In Vitro: 

    10 mM in DMSO

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3044 mL 21.5220 mL 43.0441 mL
    5 mM 0.8609 mL 4.3044 mL 8.6088 mL
    10 mM 0.4304 mL 2.1522 mL 4.3044 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay

    Cells are cultured for 24 h before drug treatment in 96 well plates. Cells were treated with Alantolactone (0, 10, 20, 30, 40, 50, and 60 μM) for 12 h and cell viability is measured by MTT assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    Mice[2]
    Female athymic BALB/c nude mice at the age of 6 weeks are used. MDA-MB-231 cells (5×106 cells/200 μL) are subcutaneously injected into the right flanks of the mice. Ten days after the injection of cells, mice are randomly divided into treatment and control groups (n=5). The animals are administered Alantolactone (2.5 mg/kg of body weight, suspended in DMSO 0.1% v/v, 100 μL i.p. injection) every 2 days, whereas control animals are treated with an equal volume of saline[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    232.32

    Formula

    C₁₅H₂₀O₂

    CAS No.

    546-43-0

    SMILES

    O=C(O[[email protected]@]1([H])[[email protected]]2([H])C=C3[[email protected]@H](C)CCC[[email protected]]3(C)C1)C2=C

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

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    Product Name:
    Alantolactone
    Cat. No.:
    HY-N0038
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    Alantolactone

    Cat. No.: HY-N0038