1. Apoptosis
    Metabolic Enzyme/Protease
  2. Apoptosis
    Endogenous Metabolite
  3. Costunolide

Costunolide (Synonyms: (+)-Costunolide; Costus lactone)

Cat. No.: HY-N0036 Purity: 99.10%
Handling Instructions

Costunolide ((+)-Costunolide) is a naturally occurring sesquiterpene lactone, with antioxidative, anti-inflammatory, antiallergic, bone remodeling, neuroprotective, hair growth promoting, anticancer, and antidiabetic properties. Costunolide can induce cell cycle arrest and apoptosis on breast cancer cells.

For research use only. We do not sell to patients.

Costunolide Chemical Structure

Costunolide Chemical Structure

CAS No. : 553-21-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 79 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 72 In-stock
Estimated Time of Arrival: December 31
10 mg USD 108 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Costunolide purchased from MCE. Usage Cited in: Gene. 2018 Dec 15;678:261-269.

    Western blot analysis of mTOR phosphorylation at Ser2448 and Ser2481 with quantification.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Costunolide ((+)-Costunolide) is a naturally occurring sesquiterpene lactone, with antioxidative, anti-inflammatory, antiallergic, bone remodeling, neuroprotective, hair growth promoting, anticancer, and antidiabetic properties. Costunolide can induce cell cycle arrest and apoptosis on breast cancer cells[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Costunolide inhibits the colony formation, migrative and invasive abilities of the H1299 cells in a dose or time dependent manner[2].
    Costunolide (6.7-215.2 μM; 24 hours) inhibits the viability of H1299 cells in a dose-dependent manner, with an IC50 of 23.93 µM[2].
    Costunolide (12.0-48.0 µM; 48 hours) induces apoptosis in H1299 cells[2].
    Costunolide (12-48.0 µM; 6-12 hours) regulates metastasis- and proliferation-associated mRNA expression[2].
    Costunolide regulates epithelial-to-mesenchymal transition (EMT)-associated protein expression[2].
    Costunolide regulates c-Myc mediated apoptosis signaling and 14-3-3-mediated signaling pathways in breast cancer cells[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: H1299 cells
    Concentration: 6.7 μM, 13.5 μM, 26.9 μM, 107.6 μM, 215.2 μM
    Incubation Time: 24 hours
    Result: Inhibited the viability of H1299 cells (MTT assay).

    Apoptosis Analysis[2]

    Cell Line: H1299 cells
    Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
    Incubation Time: 48 hours
    Result: Significantly promoted apoptosis at 24.0 µM and 48.0 µM.

    RT-PCR[2]

    Cell Line: H1299 cells
    Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
    Incubation Time: 6 hours, 12 hours
    Result: Regulated the metastasis- and proliferation-associated mRNA levels in a dose-dependent manner.

    Western Blot Analysis[2]

    Cell Line: H1299 cells
    Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
    Incubation Time: 48 hours
    Result: Significantly inhibited the EMT of H1299 cells.
    In Vivo

    Costunolide (20 mg/kg; i.p; daily; for 30 days) inhibits breast cancer through c-Myc/p53 and AKT/14-3-3 pathway[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 4 weeks old female BALB/c nude mice, MDA-MB-231 cells xenograft mouse models[3]
    Dosage: 20 mg/kg
    Administration: Intraperitoneal injection, daily, for 30 days
    Result: Reduced the expression levels of c-Myc and p-AKT and elevated the expression levels of p53 and p-14-3-3.
    Molecular Weight

    232.32

    Formula

    C₁₅H₂₀O₂

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 49 mg/mL (210.92 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3044 mL 21.5220 mL 43.0441 mL
    5 mM 0.8609 mL 4.3044 mL 8.6088 mL
    10 mM 0.4304 mL 2.1522 mL 4.3044 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.62%

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    Product Name:
    Costunolide
    Cat. No.:
    HY-N0036
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