Based on 1 Customer Validation
SB-431542 (GMP) is SB-431542 (HY-10431) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. SB-431542 is a TGF-β receptor kinase inhibitor (TRKI) in SMAD signaling.
For research use only. We do not sell to patients.
- CAS No.: 301836-41-9
- Formula: C22H16N4O3
- Molecular Weight:384.39
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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ALK4 1 μM (IC50) |
ALK5 0.75 μM (IC50) |
ALK7 2 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HaCaT | EC50 |
172 nM
Compound: 1a, SB-431542
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Inhibition of TGFbeta receptor in human HaCaT cells assessed as Smad phosphorylation by ELISA
Inhibition of TGFbeta receptor in human HaCaT cells assessed as Smad phosphorylation by ELISA
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[PMID: 20919678] |
| HEK-293T | IC50 |
0.066 μM
Compound: SB-431542, SB
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Inhibition of TGBR2 signaling in human HEK293T cells assessed as inhibition of SMAD activation after 2 to 22 hrs by dual Luciferase Assay
Inhibition of TGBR2 signaling in human HEK293T cells assessed as inhibition of SMAD activation after 2 to 22 hrs by dual Luciferase Assay
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[PMID: 23130626] |
| HepG2 | IC50 |
250 nM
Compound: SB-431542
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Inhibition of TGFR-1 in human HepG2 cells expressing PAI-luciferase by luciferase reporter gene assay
Inhibition of TGFR-1 in human HepG2 cells expressing PAI-luciferase by luciferase reporter gene assay
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[PMID: 19914068] |
| NCI-H1299 | IC50 |
0.5 μM
Compound: 3, SB-431542
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Antimigratory activity against human H1299 cells assessed as inhibition of cell migration after 12 to 24 hrs by transwell migration assay
Antimigratory activity against human H1299 cells assessed as inhibition of cell migration after 12 to 24 hrs by transwell migration assay
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[PMID: 24417479] |
| Sf9 | IC50 |
1.542 μM
Compound: 1
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Inhibition of human recombinant ALK5 phosphorylation expressed in Sf9 cells
Inhibition of human recombinant ALK5 phosphorylation expressed in Sf9 cells
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[PMID: 17552507] |
| Sf9 | IC50 |
67.1 nM
Compound: SB-431542
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Inhibition of recombinant human N-terminal GST-tagged TGFbeta1 receptor (80 to end residues) expressed in baculovirus infected Sf9 insect cells using casein as substrate measured after 2 hrs by ADP-Glo assay
Inhibition of recombinant human N-terminal GST-tagged TGFbeta1 receptor (80 to end residues) expressed in baculovirus infected Sf9 insect cells using casein as substrate measured after 2 hrs by ADP-Glo assay
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[PMID: 31408333] |
SB-431542 (GMP) (10 μM; 10 days) induces human pluripotent stem cells (hPSCs) to post-mitotic cortical neurons differentiation[1].
SB-431542 (GMP) (10 μM) facilitates directed differentiation into midbrain dopamine neurons (mDA)[2].
SB-431542 (GMP) (3.8 μM) promotes differentiation of hPSCs to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium in vitro[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human pluripotent stem cells (hPSCs)
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Concentration:10 μM
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Incubation Time:10 days
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Result:Showed downregulation of the pluripotency marker OCT4 and induction of neural and neuronal markers PAX6, FOXG1 and DCX, as well as markers of early born cortical neurons, including TBR1 (preplate, subplate and layer VI) and REELIN, in LSB+X/P/S/D conditions.
Chemical Information
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CAS No. 301836-41-9
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Appearance Solid
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Molecular Weight 384.39
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Formula C22H16N4O3
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Color White to off-white
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SMILES
O=C(C1=CC=C(C=C1)C2=NC(C3=CC=C4OCOC4=C3)=C(N2)C5=NC=CC=C5)N
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Shipping
Shipping with dry ice.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Yuchen Qi, et al. Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells. Nat Biotechnol. 2017 Feb;35(2):154-163. [Content Brief]
[2]. Tae Wan Kim, et al. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use. Cell Stem Cell. 2021 Feb 4;28(2):343-355.e5. [Content Brief]
[3]. Monica Nafria, et al. Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. STAR Protoc. 2020 Oct 16;1(3):100130. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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