2. TGF-beta/Smad
  3. TGF-β Receptor
  4. SB-431542

SB-431542 (GMP) is SB-431542 (HY-10431) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. SB-431542 is a TGF-β receptor kinase inhibitor (TRKI) in SMAD signaling.

For research use only. We do not sell to patients.

SB-431542

SB-431542 Chemical Structure

CAS No. : 301836-41-9

Size Price Stock Quantity
10 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

SB-431542 Related Antibodies

Top Publications Citing Use of Products

View All TGF-β Receptor Isoform Specific Products:

View All Isoforms
ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B BMP

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SB-431542 (GMP) is SB-431542 (HY-10431) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. SB-431542 is a TGF-β receptor kinase inhibitor (TRKI) in SMAD signaling[1][2][3].

IC50 & Target[1]

ALK4

1 μM (IC50)

ALK5

0.75 μM (IC50)

ALK7

2 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
HEK-293T IC50
0.066 μM
Compound: SB-431542, SB
Inhibition of TGBR2 signaling in human HEK293T cells assessed as inhibition of SMAD activation after 2 to 22 hrs by dual Luciferase Assay
Inhibition of TGBR2 signaling in human HEK293T cells assessed as inhibition of SMAD activation after 2 to 22 hrs by dual Luciferase Assay
[PMID: 23130626]
HaCaT EC50
172 nM
Compound: 1a, SB-431542
Inhibition of TGFbeta receptor in human HaCaT cells assessed as Smad phosphorylation by ELISA
Inhibition of TGFbeta receptor in human HaCaT cells assessed as Smad phosphorylation by ELISA
[PMID: 20919678]
HepG2 IC50
250 nM
Compound: SB-431542
Inhibition of TGFR-1 in human HepG2 cells expressing PAI-luciferase by luciferase reporter gene assay
Inhibition of TGFR-1 in human HepG2 cells expressing PAI-luciferase by luciferase reporter gene assay
[PMID: 19914068]
NCI-H1299 IC50
0.5 μM
Compound: 3, SB-431542
Antimigratory activity against human H1299 cells assessed as inhibition of cell migration after 12 to 24 hrs by transwell migration assay
Antimigratory activity against human H1299 cells assessed as inhibition of cell migration after 12 to 24 hrs by transwell migration assay
[PMID: 24417479]
Sf9 IC50
1.542 μM
Compound: 1
Inhibition of human recombinant ALK5 phosphorylation expressed in Sf9 cells
Inhibition of human recombinant ALK5 phosphorylation expressed in Sf9 cells
[PMID: 17552507]
Sf9 IC50
67.1 nM
Compound: SB-431542
Inhibition of recombinant human N-terminal GST-tagged TGFbeta1 receptor (80 to end residues) expressed in baculovirus infected Sf9 insect cells using casein as substrate measured after 2 hrs by ADP-Glo assay
Inhibition of recombinant human N-terminal GST-tagged TGFbeta1 receptor (80 to end residues) expressed in baculovirus infected Sf9 insect cells using casein as substrate measured after 2 hrs by ADP-Glo assay
[PMID: 31408333]
In Vitro

SB-431542 (GMP) (10 μM; 10 days) induces human pluripotent stem cells (hPSCs) to post-mitotic cortical neurons differentiation[1].
SB-431542 (GMP) (10 μM) facilitates directed differentiation into midbrain dopamine neurons (mDA)[2].
SB-431542 (GMP) (3.8 μM) promotes differentiation of hPSCs to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium in vitro[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SB-431542 Related Antibodies

RT-PCR[1]

Cell Line: Human pluripotent stem cells (hPSCs)
Concentration: 10 μM
Incubation Time: 10 days
Result: Showed downregulation of the pluripotency marker OCT4 and induction of neural and neuronal markers PAX6, FOXG1 and DCX, as well as markers of early born cortical neurons, including TBR1 (preplate, subplate and layer VI) and REELIN, in LSB+X/P/S/D conditions.
Molecular Weight

384.39

Formula

C22H16N4O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(C=C1)C2=NC(C3=CC=C4OCOC4=C3)=C(N2)C5=NC=CC=C5)N
Shipping

Shipping with dry ice.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

SB-431542 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SB-431542 (GMP)301836-41-9TGF-β ReceptorTransforming growth factor beta receptorsmidbrain dopamine (mDA)neuronSMADhuman pluripotent stem cells (hPSCs)Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
SB-431542
Cat. No.:
HY-10431G
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.