1. PI3K/Akt/mTOR
  2. PI3K
  3. PIK-294

PIK-294 

Cat. No.: HY-10303 Purity: 99.88%
Handling Instructions

PIK-294 is a potent p110δ-selective inhibitor with an IC50 of 10 nM.

For research use only. We do not sell to patients.

PIK-294 Chemical Structure

PIK-294 Chemical Structure

CAS No. : 900185-02-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 86 In-stock
Estimated Time of Arrival: December 31
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 140 In-stock
Estimated Time of Arrival: December 31
50 mg USD 350 In-stock
Estimated Time of Arrival: December 31
100 mg USD 550 In-stock
Estimated Time of Arrival: December 31
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Description

PIK-294 is a potent p110δ-selective inhibitor with an IC50 of 10 nM.

IC50 & Target[1]

p110δ

10 nM (IC50)

p110γ

160 nM (IC50)

p110β

490 nM (IC50)

p110α

10 μM (IC50)

In Vitro

Analysis of the specific Class I PI3 Kinase catalytic isoforms p110α (IC50=10 μM), p110β (IC50=0.49 μM), p110δ (IC50=0.01 μM) and p110γ (IC50=0.16 μM) using the inhibitor PIK-294 indicates differential roles in CXCL8-induced neutrophil migration. PIK-294 inhibits both chemokinetic and chemotactic CXCL8-induced migration[1]. When cells are pre-treated with the PI3Kδ selective inhibitor PIK-294, CXCL8-induced migration in the non-gradient and the gradient assay is significantly inhibited. PIK-294 is used at two concentrations 1 μM and 10 μM. Pre-treatment with 1 μM inhibits migration to a greater extent in the non-gradient assay than in the gradient assay. Pre-treatment with 10 μM inhibits migration to a significantly greater extent than the lower dose in both assays. Prior to stimulation with CXCL8, pre-treatment of the cells with the PI3K inhibitors, Wortmannin (50 nM), PIK-294 (10 μM) and AS-605240 (10 μM) for 2 minutes, cause a reduction in the phosphorylation of Akt. Pre-treatment of cells prior to stimulation with GM-CSF and the DMSO control with the PI3K inhibitors Wortmannin (50 nM), PIK-294 (10 μM) and AS-605240 (10 μM) for 2 minutes, reduce the phosphorylation of Akt (p<0.05 for inhibition of PI3Kδ)[2].

Molecular Weight

489.53

Formula

C₂₈H₂₃N₇O₂

CAS No.

900185-02-6

SMILES

O=C1N(C2=CC=CC=C2C)C(CN3N=C(C(C3=NC=N4)=C4N)C5=CC(O)=CC=C5)=NC6=CC=CC(C)=C16

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 40 mg/mL (81.71 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0428 mL 10.2139 mL 20.4278 mL
5 mM 0.4086 mL 2.0428 mL 4.0856 mL
10 mM 0.2043 mL 1.0214 mL 2.0428 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

Neutrophils at a concentration of 6×106 cells/mL are pre-treated with 1 μM and 10 μM of the PIK-294 for 30 mins prior to the addition of CXCL8 (100 ng/mL) or 0.5 ng/mL GM-CSF. Then a non-gradient or gradient gel assay depending on the type of migration is performed. The gels are then constructed and the migration studied[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

PIK-294PIK294PIK 294PI3KPhosphoinositide 3-kinaseInhibitorinhibitorinhibit

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PIK-294
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HY-10303
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