1. NF-κB
  2. NF-κB

(-)-DHMEQ (Synonyms: Dehydroxymethylepoxyquinomicin)

Cat. No.: HY-14645 Purity: 98.05% ee.: 100.00%
Handling Instructions

(–)-DHMEQ is a potent NF-κB inhibitor.

For research use only. We do not sell to patients.

(-)-DHMEQ Chemical Structure

(-)-DHMEQ Chemical Structure

CAS No. : 287194-40-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 241 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
2 mg USD 168 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
5 mg USD 264 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
10 mg USD 360 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
25 mg USD 720 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Other Forms of (-)-DHMEQ:

    (-)-DHMEQ purchased from MCE. Usage Cited in: Biol Pharm Bull. 2017;40(10):1669-1677.

    A549 transfectants stably expressing RelB WT (A) are preincubated with various concentrations of (–)-DHMEQ for 1 h and then incubated with or without LTβ (25 ng/mL) for 2 h in the presence or absence of (−)-DHMEQ.

    (-)-DHMEQ purchased from MCE. Usage Cited in: Biol Pharm Bull. 2017;40(10):1669-1677.

    A549 transfectants stably expressing RelB (C144S) (B) are preincubated with various concentrations of (–)-DHMEQ for 1 h and then incubated with or without LTβ (25 ng/mL) for 2 h in the presence or absence of (−)-DHMEQ.

    (-)-DHMEQ purchased from MCE. Usage Cited in: Mol Cancer Ther. 2018 Jun;17(6):1303-1314.

    Effects of TNF-α/DHMEQ on the expression of NF-κB and AR as well as neoplastic transformation of urothelial cells. Western blotting of NF-κB/p65, p-NF-κB/p65, and AR, using proteins extracted from SVHUC-AR cells cultured with ethanol, TNF-α(20 ng/mL), or DHMEQ (15 µg/mL) for 6 hours.

    (-)-DHMEQ purchased from MCE. Usage Cited in: Mol Cancer Ther. 2018 Jun;17(6):1303-1314.

    Effects of NF-κB activation/inhibition on the expression of NF-κB and its downstream targets as well as AR in bladder cancer cells. Western blotting of NF-κB/p65, p-NF-κB/p65, and AR, using proteins extracted from UMUC3 cells cultured with ethanol (mock), TNF-α (20 ng/mL), or DHMEQ (15 µg/mL) for 6 hours.

    View All NF-κB Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    (–)-DHMEQ is a potent NF-κB inhibitor.

    IC50 & Target[1]

    RelA

     

    RelB

     

    In Vitro

    (–)-DHMEQ binds to cysteine 38 of RelA and cysteine 144 of RelB. (−)-DHMEQ at concentrations greater than 25 µM inhibits the LTβ-induced nuclear translocation of FLAG-RelB WT, whereas the inhibitory effect of (–)-DHMEQ on the nuclear translocation of FLAG-RelB (C144S) became weaker at the same concentrations[1].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 3.8280 mL 19.1402 mL 38.2804 mL
    5 mM 0.7656 mL 3.8280 mL 7.6561 mL
    10 mM 0.3828 mL 1.9140 mL 3.8280 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [1]

    A549 cells transfectants stably expressing RelB WT and RelB (C144S) are preincubated with various concentrations of (-)-DHMEQ (1, 5, 10, 25, and 50 μM) for 1h and then incubated with or without LTβ (25 ng/mL) for 2 h in the presence or absence of (-)-DHMEQ.Cytoplasmic and nuclear extracts are analyzed by Western blotting[1].
    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    261.23

    Formula

    C₁₃H₁₁NO₅

    CAS No.

    287194-40-5

    SMILES

    O=C1[[email protected]@H](O2)[[email protected]@H]2[[email protected]@H](O)C(NC(C3=C(O)C=CC=C3)=O)=C1

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 32 mg/mL

    (-)-DHMEQ is prepared in DMSO and then diluted with saline[2].

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    References

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    Product Name:
    (-)-DHMEQ
    Cat. No.:
    HY-14645
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