Aldehyde reactive probe TFA

Name: Aldehyde reactive probe TFA
Brand: MedChemExpress
SKU: HY-135136
Rating: 4.5 (0 reviews)

Cat. No.: HY-135136 Purity: 97.63%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Aldehyde reactive probe TFA is an aldehyde reactive probe. Aldehyde reactive probe TFA specifically labels the AP site in DNA with biotin residues. Aldehyde reactive probe TFA can detect RNA oxidation-induced debasement sites. Aldehyde reactive probe TFA can be used to quantify AP sites or detect other aldehyde-containing DNA damage and RNA oxidation-induced debasement sites.

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Aldehyde reactive probe TFA 화학구조

CAS No. : 627090-10-2

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	견적 받기
200 mg	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of Aldehyde reactive probe TFA:

ARP In-stock

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

Aldehyde reactive probe TFA (100 μL; 1 h at 37 °C) detects AP sites and related aldehyde-containing damages in DNA isolated from heat-inactivated, X-irradiated E. coli AB1157 cells, with signal intensity proportional to radiation dose^[1].
Aldehyde reactive probe TFA (100 μL; 1 h at 37 °C) detects AP sites formed in the DNA of MMS-treated E. coli AB1157 cells. Its signal is proportional to the concentration of MMS and decreases during the repair process after treatment^[1].
Aldehyde reactive probe TFA (2 mM; 1 h) specifically reacts with apurinic sites in acid-apurinized yeast tRNA^Phe, and its reaction activity is directly proportional to the degree of apurinization^[2].
Aldehyde reactive probe TFA (0-10 mM; 1 h) specifically binds to abasic sites in chemically synthesized RNA, with a limit of detection of 10 fmoles of abasic sites^[2].
The reactivity of Aldehyde reactive probe TFA (0-10 mM; 0.5-2 h) with synthetic abasic RNA increases with elevated temperature, prolonged incubation time up to 1 h, acidic pH, and increased ARP concentration^[2].
Aldehyde reactive probe TFA (2 mM; 1 h) detects apurinic/apyrimidinic sites in in vitro synthesized RNA irradiated with γ-rays in a dose-dependent manner, and this effect occurs at doses ≥5 Gy^[2].
Aldehyde reactive probe TFA (2 mM; 1 h) detects apurinic/apyrimidinic (AP) sites in in vitro synthesized RNA oxidized by the Fenton reaction in a dose-dependent manner, and covalently binds to abasic glycosyl groups in Fenton reaction-oxidized RNA to form ribose-ARP adducts detectable by LC/MS^[2].
Aldehyde reactive probe TFA (2 mM; 1 h) detects apurinic/apyrimidinic sites in in vitro synthesized RNA oxidized by peroxynitrite in a dose-dependent manner^[2].
Aldehyde reactive probe TFA (2 mM; 1 h) detected an increase in the formation of abasic sites in the total RNA of HeLa cells treated with hydrogen peroxide or peroxynitrite^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

445.41

화학식

C₁₄H₂₂F₃N₅O₆S

CAS No.

627090-10-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(NNC(CON)=O)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O.O=C(O)C(F)(F)F

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도

In Vitro:

DMSO : 25 mg/mL (56.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2451 mL	11.2256 mL	22.4512 mL
5 mM	0.4490 mL	2.2451 mL	4.4902 mL
10 mM	0.2245 mL	1.1226 mL	2.2451 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 97.63%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (248 KB) HNMR (194 KB) RP-HPLC (242 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Kubo K, et al. A novel, sensitive, and specific assay for abasic sites, the most commonly produced DNA lesion. Biochemistry. 1992;31(14):3703-3708. [Content Brief]

[2]. Tanaka M, et al. An assay for RNA oxidation induced abasic sites using the Aldehyde Reactive Probe. Free Radic Res. 2011;45(2):237-247. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2451 mL	11.2256 mL	22.4512 mL	56.1281 mL
	5 mM	0.4490 mL	2.2451 mL	4.4902 mL	11.2256 mL
	10 mM	0.2245 mL	1.1226 mL	2.2451 mL	5.6128 mL
	15 mM	0.1497 mL	0.7484 mL	1.4967 mL	3.7419 mL
	20 mM	0.1123 mL	0.5613 mL	1.1226 mL	2.8064 mL
	25 mM	0.0898 mL	0.4490 mL	0.8980 mL	2.2451 mL
	30 mM	0.0748 mL	0.3742 mL	0.7484 mL	1.8709 mL
	40 mM	0.0561 mL	0.2806 mL	0.5613 mL	1.4032 mL
	50 mM	0.0449 mL	0.2245 mL	0.4490 mL	1.1226 mL