Anti-Mouse TIM-4 Antibody (RMT4-53)
Based on 1 Customer Validation
Anti-Mouse TIM-4 Antibody (RMT4-53) is a rat-derived IgG2b κ type antibody inhibitor, targeting to mouse TIM-4. Anti-Mouse TIM-4 Antibody (RMT4-53) can block T cell immunoglobulin and mucin domain 4 (TIM-4). Anti-Mouse TIM-4 Antibody (RMT4-53) can inhibit the engulfment of apoptotic cells and T cell proliferation. Anti-Mouse TIM-4 Antibody (RMT4-53) can be used for the researches of cancer, immunology and metabolic disease, such as MC38 tumor and graft.
For research use only. We do not sell to patients.
- Purity : 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2b kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
TIM-4
In Vitro
Anti-Mouse TIM-4 Antibody (RMT4-53) (2-4 h) inhibits T cell STAT6 activation in CD4+ T cells [1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (4 h) inhibits phagocytosis of apoptotic EG7 cells in Timd4−/− and WT peritoneal macrophages[2].
Anti-Mouse TIM-4 Antibody (RMT4-53) (4 h) augments cross-priming of OVA-specific T cells in TIM-4+ bone-marrow derived macrophages (BMDMs)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse TIM-4 Antibody (RMT4-53) (500 μg at days 0 and 250 μg at days 2, 4, 6 and 8, i.p.) prolongs graft survival in B6 CD4+25- T cells transferred B6 RAG−/− mice of a bm12 skin transplant[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (i.p., at days 8, 10 and 12) inhibits tumor growth companied with CDDP (HY-17394) in MC38 tumor Atg5flox/flox mice[2].
Anti-Mouse TIM-4 Antibody (RMT4-53) (0.5 mg/mouse, i.v., 1 h prior to ischemia) suppresses liver damage in segmental (70%) hepatic ischemia-reperfusion injury (IRI) mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:B6 CD4+25- T cells transferred B6 RAG−/− mice of a bm12 skin transplant[1]
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Dosage:500 μg at days 0 and 250 μg at days 2, 4, 6 and 8
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Administration:Intraperitoneally injection
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Result:Prolonged graft survival.
Increased the generation of iTregs.
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Animal Model:Segmental (70%) hepatic ischemia-reperfusion injury (IRI) mice[3]
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Dosage:0.5 mg/mouse
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Administration:Intravenously injection, 1 h prior to ischemia
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Result:Decreased sALT levels.
Ameliorated histological features of liver IRI.
Gene ID
Accession
Q6U7R4 / NP_848874.3
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo TIM-4 blockade; in vitro TIM-4 blockade; Immunofluorescence
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse TIM-4 Antibody (RMT4-53)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yeung MY, et al. Interruption of dendritic cell-mediated TIM-4 signaling induces regulatory T cells and promotes skin allograft survival. J Immunol. 2013 Oct 15;191(8):4447-55. [Content Brief]
[2]. Baghdadi M, et al. TIM-4 glycoprotein-mediated degradation of dying tumor cells by autophagy leads to reduced antigen presentation and increased immune tolerance. Immunity. 2013 Dec 12;39(6):1070-81. [Content Brief]
[3]. Ji H, et al. T-cell immunoglobulin and mucin domain 4 (TIM-4) signaling in innate immune-mediated liver ischemia-reperfusion injury. Hepatology. 2014 Dec;60(6):2052-2064. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse TIM-4 Antibody (RMT4-53) Related Classifications
HY-P990820 Related Classifications
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