Anti-TROP-2 Antibody (Pr1E11) 

Anti-TROP-2 Antibody (Pr1E11) is a kind of mouse IgG1 κ chimeric antibody inhibitor, targeting to human TROP-2. Anti-TROP-2 Antibody (Pr1E11) specifically binds to TROP-2 with high affinity and shows low internalization activitv. Anti-TROP-2 Antibody (Pr1E11) can be used for the research of cancer, such as epithelial cancer and primary pancreatic cancer^[1].

Mouse IgG1 kappa

Mouse IgG1 kappa, Isotype Control

Human

TROP-2

Anti-TROP-2 Antibody (Pr1E11) (defucosylated [Fuc(−)] chimeric Pr1E11) (0.1-1,000 ng/mL, 4 h) shows antibody-dependent cellular cytotoxicity (ADCC) activity but not complement-dependent cytotoxicity (CDC) activity in BxPC3 and Colo205 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-TROP-2 Antibody (Pr1E11) (defucosylated [Fuc(−)] chimeric Pr1E11) (200 μg, i.p., twice a week for 4 weeks) shows anti-tumor effect in BxPC3/Colo205 tumor SCID mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

56753  [NCBI]

Q8BGV3

Western blot; Immunohistochemistry (frozen); Flow cytometry

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-TROP-2 Antibody (Pr1E11)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1×10⁶ MCF-7 cells with Anti-TROP-2 Antibody (Pr1E11) (HY-P990827, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa Isotype Control (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Ikeda M, et al. Pr1E11, a novel anti-TROP-2 antibody isolated by adenovirus-based antibody screening, recognizes a unique epitope. Biochem Biophys Res Commun. 2015 Mar 20;458(4):877-82.  [Content Brief]