AF488-conjugated CD16 Antibody (3G8)
(Synonyms: CD16,CD16-AF488,3G8)AF488-conjugated CD16 Antibody (3G8) is a Mouse-derived and AF488 conjugated monoclonal antibody, targeting to CD16.
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Host:
Mouse
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Isotype:
Mouse IgG1,κ
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Conjugation:
AF488
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 5 ul per 106 cells in a 100 µl suspension |
Product Details
AF488-conjugated CD16 Antibody (3G8) is a Mouse-derived and AF488 conjugated monoclonal antibody, targeting to CD16.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
AF488
Unmodified
Mouse IgG1,κ
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Storage & Stability
Stored at 2-8°C for 1 year, protect from light, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CD16 (FcγRIII) is a low-affinity IgG Fc receptor, including two isoforms: CD16A (FCGR3A) and CD16B (FCGR3B). CD16A is mainly expressed on NK cells, monocytes, and macrophages, and mediates ITAM signaling through the FcRγ chain, playing roles such as antibody-dependent cytotoxicity (ADCC), phagocytosis, and inflammatory activation. CD16B is mainly expressed on neutrophils and participates in the binding and clearance of IgG immune complexes. CD16 has important value in tumor immunology, antibody drug development, infection, and autoimmune disease research.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Secreted
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Expression
Tissue_specificity:Expression (protein level) in natural killer cells (PubMed: 2526846) . Expression (protein level) in some circulating monocytes (PubMed: 27670158) .
Induction:Up-regulated in a monocyte subset upon exposure to IL10, TGFB and MCSF
Positive sample: Human peripheral blood granulocytes. -
Subunit
Forms a heterooligomeric complex with ITAM-containing signaling subunits, either a homodimer of CD247, a homodimer of FCER1G or a heterodimer of CD247 and FCER1G. Interacts (via transmembrane domain) with signaling subunits; this interaction is a prerequisite for receptor complex expression on the cell surface and intracellular signal transduction. Binds the Fc region of antigen-complexed IgG with a preference for IgG1 and IgG3 isotypes. Interacts with CD2; this interaction is involved in NK cell activation and cytotoxicity. Interacts with S100A4; this interaction inhibits PKC-dependent phosphorylation of FCGR3A.
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Synonyms
CD16,CD16-AF488,3G8
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