AF488-conjugated CD19 Antibody (4G7)
(Synonyms: CD19,CD19-AF488,4G7)AF488-conjugated CD19 Antibody (4G7) is a Mouse-derived and AF488 conjugated monoclonal antibody, targeting to CD19.
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Host:
Mouse
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Isotype:
Mouse IgG1,κ
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Conjugation:
AF488
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 5 ul per 106 cells in a 100 µl suspension |
Product Details
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Storage & Stability
Stored at 2-8°C for 1 year, protect from light, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CD19 is a B cell-specific transmembrane glycoprotein and a core member of the B cell receptor (BCR) co-receptor complex. It can significantly enhance BCR signaling by activating signaling pathways such as PI3K and BTK, promoting B cell development, activation, and antibody production. CD19 is present throughout almost the entire B cell development process and is a biomarker for all B cells. CD19 is an important marker for immunophenotyping of B-cell leukemia and lymphoma, and is also one of the targets for CAR-T cell therapy, bispecific antibodies, and other B cell-targeted therapies.
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Expression
Tissue_specificity:Detected in B cells in the marginal zone of lymph nodes and germinal centers (PubMed:2463100) . Detected in blood B cells (protein levels) (PubMed:16672701, PubMed:2463100) . -
Isoforms & Post-Translational Modification
CD19 has 2 isomers: P15391-1: 61128 Da (predicted); P15391-2: 61200 Da (predicted).
Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. -
Subunit
Interacts with CR2/CD21. Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells. Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN. Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2). Interacts with GRB2
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Synonyms
CD19,CD19-AF488,4G7
Documentation
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