AF488-conjugated CD3 Antibody (SK7)
(Synonyms: CD3,CD3-AF488,SK7)AF488-conjugated CD3 Antibody (SK7) is a Mouse-derived and AF488 conjugated monoclonal antibody, targeting to CD3 .
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Host:
Mouse
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Isotype:
Mouse IgG1,κ
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Conjugation:
AF488
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 5 ul per 106 cells in a 100 µl suspension |
Product Details
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.2) containing 0.1% Proclin300 and protein stabilizer.
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Storage & Stability
Stored at 2-8°C for 1 year, protect from light, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells) . It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Peroxisome
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Expression
Tissue_specificity:Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells -
Isoforms & Post-Translational Modification
CD3 has 3 isomers: P20138-1: 39825 Da (predicted); P20138-2: 33890 Da (predicted); P20138-3: 25293 Da (predicted).
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition;Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent -
Subunit
Homodimer; disulfide-linked (PubMed:10887109). Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation (PubMed:10206955, PubMed:10556798).
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Synonyms
CD3,CD3-AF488,SK7
Documentation
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