Copy Link Print Information

alpha 1 Antitrypsin Antibody (YA1122)

(Synonyms: SERPINA1; AAT; PI; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1)
Customer Review

Based on 1 Customer Validation

alpha 1 Antitrypsin Antibody (YA1122) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha 1 Antitrypsin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL IHC-P mIHC
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:100-1:200

Product Details

Description

alpha 1 Antitrypsin Antibody (YA1122) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha 1 Antitrypsin.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database

Entrez Gene: 5265 Human

SwissProt: P01009 Human

Immunogen

Recombinant protein of human alpha 1 Antitrypsin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103492

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Endometrial Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    alpha 1 Antitrypsin is an Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin; Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE)

  • Subcellular Localization

    Secreted; Endoplasmic reticulum; Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:It is ubiquitous. It is expressed in both leukocytes and plasma.

  • Isoforms & Post-Translational Modification

    P01009 has 3 isomers: P01009-1: 46737 Da (predicted); P01009-2: 40263 Da (predicted); P01009-3: 34755 Da (predicted).
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant;Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418;(Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases

  • Subunit

    Interacts with CELA2A (PubMed:31358993). Interacts with ERGIC3 and LMAN1/ERGIC53 (PubMed:31142615). Interacts with PRSS1/trypsin (PubMed:11057674). Interacts with PRSS1/Trypsin (PubMed:11057674). The variants S and Z interact with CANX and PDIA3 (PubMed:23826168)

  • SwissProt ID

    P01009

  • Gene ID
    5265 [NCBI]
  • Synonyms

    SERPINA1; AAT; PI; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1

  • Research Field

    Cell Biology

alpha 1 Antitrypsin Antibody (YA1122) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Endometrial Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    1/16
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Endometrial Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • Experimental Validation Results for alpha 1 Antitrypsin Antibody (YA1122)
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using alpha 1 Antitrypsin antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Endometrial Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Kidney cancer tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using alpha 1 Antitrypsin antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81377, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • alpha 1 Antitrypsin Antibody (YA1122) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image IHC-P
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image mIHC
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image mIHC
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image mIHC
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image mIHC
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image mIHC
  • alpha 1 Antitrypsin Antibody (YA1122) Product Image mIHC