ARL3 Antibody (YA8695)

Cat. No.: HY-P89011: Ficha de datos User Guide for Antibodies
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ARL3 Antibody (YA8695) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ARL3.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

ARL3 Antibody (YA8695) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ARL3.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 20.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P36405

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-182 of human ARL3 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed:16525022, PubMed:18588884). Required for normal cytokinesis and cilia signaling (PubMed:22085962). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E (PubMed:30269812). Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (PubMed:22085962). Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium (By similarity)

Subcellular Localization：Golgi apparatus membrane,Cytoplasm, cytoskeleton, spindle,Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm,Cell projection, cilium

Expression：
Tissue_Specificity: Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level)

Isoforms & Post-Translational Modification：P36405: 182 amino acids, molecular weight 20456 Da.

Subunit：Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B) (PubMed:11303027, PubMed:11847227, PubMed:18588884)

Synonyms

ARFL3

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/sf9-cell.html [Content Brief]