ASB2 Antibody (YA8650)(PBS only)

Cat. No.: HY-P88966A: Data Sheet User Guide for Antibodies
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ASB2 Antibody (YA8650) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASB2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ASB2 Antibody (YA8650) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASB2.

Host

Mouse

Molecular Weight

Predicted band size: 64.9 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96Q27

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-300 of human ASB2 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15590664, PubMed:16325183). Mediates Notch-induced ubiquitination and degradation of substrates including TCF3/E2A and JAK2 (PubMed:21119685). Required during embryonic heart development for complete heart looping (By similarity). Required for cardiomyocyte differentiation (PubMed:32179481). Specifically promotes the ubiquitination of SMAD9 and targets it for proteasomal degradation, leading to avoid excessive accumulation of SMAD9 (PubMed:34845242). Plays a role in the regulation of NK-cell migration by modulating protein levels of filamin A/FLNA via regulation of its ubiquitination and proteasome degradation (By similarity)|Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA (PubMed:19300455). Also targets DES for proteasomal degradation (By similarity). Acts as a negative regulator of skeletal muscle mass (By similarity)|Targets filamins FLNA and FLNB for proteasomal degradation (PubMed:21737450, PubMed:22916308, PubMed:24044920, PubMed:24052262). This leads to enhanced adhesion of hematopoietic cells to fibronectin (PubMed:21737450). Required for FLNA degradation in immature cardiomyocytes which is necessary for actin cytoskeleton remodeling, leading to proper organization of myofibrils and function of mature cardiomyocytes (By similarity). Required for degradation of FLNA and FLNB in immature dendritic cells (DC) which enhances immature DC migration by promoting DC podosome formation and DC-mediated degradation of the extracellular matrix (By similarity). Does not promote proteasomal degradation of tyrosine-protein kinases JAK1 or JAK2 in hematopoietic cells (PubMed:22916308)

Subcellular Localization：Cytoplasm, cytoskeleton, stress fiber,Cytoplasm, myofibril, sarcomere, Z line

Expression：
Tissue_Specificity: Expressed in muscle cells|Expressed in hematopoietic cells
Induction: Induced by all-trans retinoic acid (ATRA) (PubMed:11566180, PubMed:11682484). Induced by Notch signaling (PubMed:21119685). Repressed by microRNA miR-29c (PubMed:29955039)

Isoforms & Post-Translational Modification：Q96Q27 has two isomers: Q96Q27-2: 70212 Da (predicted); Q96Q27-1: 65084 Da (predicted).
Monoubiquitinated丨Not monoubiquitinated丨Phosphorylation at Ser-371 is required for association with FLNA and subsequent FLNA degradation

Subunit：Component of a probable ECS E3 ubiquitin-protein ligase complex which contains CUL5, either RBX1 or RNF7/RBX2, Elongin BC complex (ELOB and ELOC) and ASB2 (PubMed:15590664, PubMed:16325183, PubMed:19300455, PubMed:21119685, PubMed:24044920)

Synonyms

ASB-2

Documentation

User Guide for Antibodies (1077 KB)