Beclin 1 Antibody (YA6433)

Cat. No.: HY-P86741: Data Sheet User Guide for Antibodies
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Beclin 1 Antibody (YA6433) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Beclin 1 Antibody (YA6433) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 50 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q14457

Gene ID

8678 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human Beclin 1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense|Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors|(Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus

Subcellular Localization：Cytoplasm,Golgi apparatus, trans-Golgi network membrane,Endosome membrane,Endoplasmic reticulum membrane,Mitochondrion membrane,Endosome,Cytoplasmic vesicle, autophagosome,Mitochondrion,Nucleus,Cytoplasm,Mitochondrion

Expression：
Tissue_Specificity: Ubiquitous

Subunit：A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity)

Synonyms

Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.

Documentation

User Guide for Antibodies (1077 KB)