CD28 Antibody (YA1092)(PBS only)

Cat. No.: HY-P81347A: Data Sheet User Guide for Antibodies
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CD28 Antibody (YA1092) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD28.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD28 Antibody (YA1092) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD28.

Host

Rabbit

Molecular Weight

Predicted band size: 16.5 kDa;

Observed band size: 16.5 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P10747

Gene ID

940 [NCBI]

Immunogen

A synthesized peptide derived from human CD28

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:2000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed:1650475, PubMed:7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed:24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed:1650475, PubMed:35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed:21964608, PubMed:24665965, PubMed:7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed:8617933); Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell surface

Expression：
Tissue_specificity:Expressed in T-cells and plasma cells, but not in less mature B-cells

Isoforms & Post-Translational Modification：P10747 has 7 isomers: P10747-1: 25066 Da (predicted); P10747-2: 11528 Da (predicted); P10747-3: 15369 Da (predicted); P10747-4: 14014 Da (predicted); P10747-5: 6099 Da (predicted); P10747-6: 8823 Da (predicted); P10747-7: 26186 Da (predicted).
Phosphorylated by LCK (PubMed:7568038). Dephosphorylated by PTPN11 (PubMed:36114179);Tyrosine phosphorylated induced by CD40LG

Subunit：Homodimer; disulfide-linked (PubMed:35397202). Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70 (PubMed:12196291). Interacts with GRB2 (PubMed:24098653, PubMed:7568038). Interacts with PIK3R1 (PubMed:7568038). Interacts with PRKCQ (PubMed:21964608)

Research Field

Immunology

Synonyms

CD28; T-cell-specific surface glycoprotein CD28; TP44; CD28

Documentation

User Guide for Antibodies (1077 KB)