CD48 Antibody (YA1111)(PBS only)

Cat. No.: HY-P81366A: Ficha de datos User Guide for Antibodies
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CD48 Antibody (YA1111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD48.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

CD48 Antibody (YA1111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD48.

Host

Rabbit

Peso molecular

Predicted band size: 28 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P09326

Gene ID

962 [NCBI]

Immunogen

A synthesized peptide derived from human CD48

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Pureza	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:19494291, PubMed:27249817, PubMed:9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817)

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Membrane raft; Secreted

Expression：
Tissue_specificity:Widely expressed in all hematopoietic cells

Induction:By IFN-alpha/beta and IFN-gamma both at the level of CD48 mRNA and cell surface expression

Isoforms & Post-Translational Modification：P09326 has 2 isomers: P09326-1: 27683 Da (predicted); P09326-2: 19429 Da (predicted).

Subunit：Interacts with CD2 (PubMed:19494291). Interacts with CD244; this interaction is possible not only on different cells (trans interaction) but also on the same cell (cis interaction) (PubMed:27249817, PubMed:9841922). Interacts with LCK (PubMed:12007789)

Research Field

Immunology

Synonyms

CD48; BCM1; BLAST1; CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; TCT.1; CD48

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/a-431-cell.html [Content Brief]