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CRX Antibody (YA1184)

(Synonyms: Cone rod homeobox; CORD 2; CRD; CRX)
Customer Review

Based on 1 Customer Validation

CRX Antibody (YA1184) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRX.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P, mIHC

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.19.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CRX Antibody (YA1184)
  • Experimental Validation Results for CRX Antibody (YA1184)
  • CRX Antibody (YA1184) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:50-1:200 1:50-200

Product Details

Description

CRX Antibody (YA1184) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRX.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database

Entrez Gene: 1406 Human

SwissProt: O43186 Human

Immunogen

A synthesized peptide derived from human CORD2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103554

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.19.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRX Antibody (YA1184)
    Immunohistochemical analysis of paraffin-embedded human Rat eye globe tissue using CRX antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CRX Antibody (YA1184)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Rat eye globe tissue using CRX antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×) (HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    CRX transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, such as NRL, RORB and RAX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Retina

  • Subunit

    Interacts (via the homeobox) with NRL (via the leucine-zipper domain). Interacts with PDC, RAX2, RORB and SCA7

  • SwissProt ID

    O43186

  • Gene ID
    1406 [NCBI]
  • Synonyms

    Cone rod homeobox; CORD 2; CRD; CRX

  • Research Field

    Neuroscience

CRX Antibody (YA1184) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Rat eye globe tissue using CRX antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Rat eye globe tissue using CRX antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×) (HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • CRX Antibody (YA1184) Product Image
    1/16
  • Experimental Validation Results for CRX Antibody (YA1184)
  • Experimental Validation Results for CRX Antibody (YA1184)
  • CRX Antibody (YA1184) Product Image
  • Experimental Validation Results for CRX Antibody (YA1184)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Rat eye globe tissue using CRX antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CRX Antibody (YA1184)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Rat eye globe tissue using CRX antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×) (HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for CRX Antibody (YA1184)
  • Experimental Validation Results for CRX Antibody (YA1184)
  • CRX Antibody (YA1184) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Rat eye globe tissue using CRX antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CRX Antibody (YA1184) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Rat eye globe tissue using CRX antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81439, 1:50 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×) (HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • CRX Antibody (YA1184) Product Image IHC-P
  • CRX Antibody (YA1184) Product Image mIHC