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CYTH2 Antibody (YA7905)

(Synonyms: ARNO; CTS18; CTS18.1; cytohesin-2; PSCD2; PSCD2L; SEC7L; Sec7p-L; Sec7p-like)

CYTH2 Antibody (YA7905) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYTH2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • CYTH2 Antibody (YA7905) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:150-500

Product Details

Description

CYTH2 Antibody (YA7905) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYTH2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 46.4 kDa
Immunogen

Full length human recombinant protein of human CYTH2 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human pancreas using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human kidney using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human lung cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human liver cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human colon cacner using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    Immunohistochemical analysis of paraffin-embedded human liver using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CYTH2 acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Involved in neurite growth[1].

  • Subcellular Localization

    Cell membrane,Cytoplasm,Cell projection,Cell projection, growth cone,Cell junction, tight junction,Cell junction, adherens junction

  • Expression


    Tissue_Specificity: Widely expressed

  • Isoforms & Post-Translational Modification

    Q99418 has two isomers: Q99418-1: 46546 Da (predicted); Q99418-2: 46489 Da (predicted).

  • Subunit

    Heteromer

  • SwissProt ID

    Q99418

  • Synonyms

    ARNO; CTS18; CTS18.1; cytohesin-2; PSCD2; PSCD2L; SEC7L; Sec7p-L; Sec7p-like

CYTH2 Antibody (YA7905) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cacner using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    1/16
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • CYTH2 Antibody (YA7905) Product Image
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cacner using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • Experimental Validation Results for CYTH2 Antibody (YA7905)
  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cacner using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver using CYTH2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88221, 1/150) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYTH2 Antibody (YA7905) Product Image IHC-P
  • CYTH2 Antibody (YA7905) Product Image IHC-P
  • CYTH2 Antibody (YA7905) Product Image IHC-P
  • CYTH2 Antibody (YA7905) Product Image IHC-P
  • CYTH2 Antibody (YA7905) Product Image IHC-P
  • CYTH2 Antibody (YA7905) Product Image IHC-P
  • CYTH2 Antibody (YA7905) Product Image IHC-P