EHHADH Antibody (YA8059)
(Synonyms: ECHD; FRTS3; L-PBE; LBFP; LBP; PBFE)EHHADH Antibody (YA8059) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EHHADH.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB, IHC-P
-
Reactivity :
Human
-
Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
|---|---|---|
| Dilution Ratio | 1:1000-4000 | 1:150-500 |
Product Details
EHHADH Antibody (YA8059) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EHHADH.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Calculated Molecular Weight Predicted band size: 79.3 kDa
Human recombinant protein fragment corresponding to amino acids 496-723 of human EHHADH produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Verification Images
-
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using EHHADH Antibody (HY-P88375, 1/150). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 15 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using EHHADH Antibody (HY-P88375, 1/150). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 15 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using EHHADH Antibody (HY-P88375, 1/150). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 15 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using EHHADH Antibody (HY-P88375, 1/150). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 15 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
-
Function
EHHADH is a Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway. Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways. Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation[1].
-
Subcellular Localization
Peroxisome
-
Expression
Tissue_Specificity: Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain -
Isoforms & Post-Translational Modification
Q08426 has two isomers: Q08426-1: 79495 Da (predicted); Q08426-2: 69154 Da (predicted).
Acetylated, leading to enhanced enzyme activity -
Subunit
Monomer
-
SwissProt ID
-
Synonyms
ECHD; FRTS3; L-PBE; LBFP; LBP; PBFE
Documentation
Powered by Bioz
