KMT5A Antibody (YA9672)
(Synonyms: PR-Set7, SET07, SET8, SETD8)KMT5A Antibody (YA9672) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to KMT5A.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:150 |
Product Details
KMT5A Antibody (YA9672) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to KMT5A.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 39 kDa;
Entrez Gene: 387893
Recombinant protein of human SETD8(Full length) produced in E.coli.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
KMT5A is a Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Induction: By HCFC1 C-terminal chain, independently of HCFC1 N-terminal chain. Transiently induced by TGF-beta and during the cell cycle. -
Isoforms & Post-Translational Modification
KMT5A has 2 isoforms, Q9NQR1-1: amino acid length is 393, molecular weight is 42890 Da (predicted); Q9NQR1-2: amino acid length is 352, molecular weight is 39223 Da (predicted).
Acetylated at Lys-162; does not affect methyltransferase activity. Deacetylated at Lys-162 possibly by SIRT2; does not change methyltransferase activity -
Subunit
Interacts with L3MBTL1
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SwissProt ID
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Synonyms
PR-Set7, SET07, SET8, SETD8
Documentation
References
[1]. Nishioka K, et al. PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin. Mol Cell. 2002 Jun;9(6):1201-13. [Content Brief]
[2]. Fang J, et al. Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase. Curr Biol. 2002 Jul 9;12(13):1086-99. [Content Brief]
[3]. Yin Y, et al. SET8 recognizes the sequence RHRK20VLRDN within the N terminus of histone H4 and mono-methylates lysine 20. J Biol Chem. 2005 Aug 26;280(34):30025-31. [Content Brief]
[4]. Shi X, et al. Modulation of p53 function by SET8-mediated methylation at lysine 382. Mol Cell. 2007 Aug 17;27(4):636-46. [Content Brief]
[5]. Saredi G, et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature. 2016 Jun 30;534(7609):714-718. [Content Brief]
[6]. Julien E, et al. A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1. Mol Cell. 2004 Jun 18;14(6):713-25. [Content Brief]
[7]. Xiao B, et al. Specificity and mechanism of the histone methyltransferase Pr-Set7. Genes Dev. 2005 Jun 15;19(12):1444-54. [Content Brief]
[8]. Couture JF, et al. Structural and functional analysis of SET8, a histone H4 Lys-20 methyltransferase. Genes Dev. 2005 Jun 15;19(12):1455-65. [Content Brief]
[9]. Sims JK, et al. A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin. J Biol Chem. 2006 May 5;281(18):12760-6. [Content Brief]
[10]. Abbas T, et al. CRL1-FBXO11 promotes Cdt2 ubiquitylation and degradation and regulates Pr-Set7/Set8-mediated cellular migration. Mol Cell. 2013 Mar 28;49(6):1147-58. [Content Brief]
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