LIME1 Antibody (YA8724)

Cat. No.: HY-P89040: Data Sheet User Guide for Antibodies
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LIME1 Antibody (YA8724) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LIME1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

LIME1 Antibody (YA8724) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LIME1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 31.1 kDa

Species Reactivity

Human

SwissProt ID

Q9H400

Immunogen

Human recombinant protein fragment corresponding to amino acids 30-295 of human LIME1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules

Subcellular Localization：Cell membrane

Expression：
Tissue_Specificity: Expressed in peripheral blood lymphocytes, lymphoid tissues, and liver. Present in T-cells and plasma cells, and in various hematopoietic cell lines (at protein level)

Isoforms & Post-Translational Modification：Q9H400 has two isomers: Q9H400-1: 31288 Da (predicted); Q9H400-2: 20841 Da (predicted).
Palmitoylation of Cys-28 and Cys-31 is required for raft targeting丨Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins

Subunit：When phosphorylated in response to BCR activation, interacts with LYN, PIK3R1, PLCG2, and GRB2 (By similarity)

Synonyms

dJ583P15.4; LIME

Documentation

User Guide for Antibodies (1077 KB)