MAP4K4/NIK Antibody (YA1123)(PBS only)

Cat. No.: HY-P81378A: Data Sheet User Guide for Antibodies
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MAP4K4/NIK Antibody (YA1123) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAP4K4/NIK.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MAP4K4/NIK Antibody (YA1123) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAP4K4/NIK.

Host

Rabbit

Molecular Weight

Predicted band size: 142 kDa;

Observed band size: 142 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95819

Gene ID

9448 [NCBI]

Immunogen

A synthesized peptide derived from human MAP4K4

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway (PubMed:9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). Phosphorylates SMAD1 on Thr-322 (PubMed:21690388)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Widely expressed. Isomer 5 is abundant in the brain. Isomer 4 is mainly found in the liver, skeletal muscle, and placenta.

Isoforms & Post-Translational Modification：O95819 has 6 isomers: O95819-1: 142101 Da (predicted); O95819-2: 138505 Da (predicted); O95819-3: 151000 Da (predicted); O95819-4: 133401 Da (predicted); O95819-5: 141929 Da (predicted); O95819-6: 145005 Da (predicted).

Subunit：Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially)

Research Field

Signal Transduction

Synonyms

MAP4K4; HGK, KIAA0687, NIK; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4 (MEK kinase kinase 4; MEKKK 4); Nck-interacting kinase

Documentation

User Guide for Antibodies (1077 KB)