MARK4 Antibody (YA7937)(PBS only)

Cat. No.: HY-P88253A: Data Sheet User Guide for Antibodies
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MARK4 Antibody (YA7937) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MARK4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MARK4 Antibody (YA7937) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MARK4.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96L34

Immunogen

Human recombinant protein fragment corresponding to amino acids 390-467 of human MARK4 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase (PubMed:14594945, PubMed:15009667, PubMed:23184942, PubMed:23666762). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942). Involved in NLRP3 positioning along microtubules by mediating NLRP3 recruitment to microtubule organizing center (MTOC) upon inflammasome activation (PubMed:28656979)

Subcellular Localization：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm, cytoskeleton, microtubule organizing center,Cytoplasm, cytoskeleton, cilium basal body,Cytoplasm, cytoskeleton, cilium axoneme,Cytoplasm,Cell projection, dendrite

Expression：
Tissue_Specificity: Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945)

Isoforms & Post-Translational Modification：Q96L34 has two isomers: Q96L34-1: 82520 Da (predicted); Q96L34-2: 75321 Da (predicted).
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation丨Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552)

Subunit：Interacts with MAPT/TAU (PubMed:23666762)

Synonyms

MARK4L; MARK4S; MARKL1; MARKL1L; PAR-1D

Documentation

User Guide for Antibodies (1077 KB)