MCF2 Antibody (YA8841)(PBS only)

Cat. No.: HY-P89157A: Data Sheet User Guide for Antibodies
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MCF2 Antibody (YA8841) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCF2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MCF2 Antibody (YA8841) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCF2.

Host

Mouse

Species Reactivity

Human

SwissProt ID

P10911

Immunogen

Human recombinant protein fragment corresponding to amino acids 872-1001 of human MCF2 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42

Subcellular Localization：Cytoplasm,Membrane,Membrane

Expression：
Tissue_Specificity: Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis

Isoforms & Post-Translational Modification：P10911 has 6 isomers: P10911-1: 107673 Da (predicted); P10911-2: 100409 Da (predicted); P10911-3: 114376 Da (predicted); P10911-4: 109436 Da (predicted); P10911-5: 116139 Da (predicted); P10911-6: 95940 Da (predicted).
Phosphorylation by TNK2 enhances guanine nucleotide exchange factor (GEF) activity toward Rho family proteins

Subunit：Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P)

Synonyms

ARHGEF21; DBL

Documentation

User Guide for Antibodies (1077 KB)