p53R2 Antibody
(Synonyms: P53R2, RRM2B, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2)p53R2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to p53R2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol and 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:100-500 | 1:50-100 | 1:1000-2000 |
Product Details
p53R2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to p53R2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 41 kDa
Recombinant protein of C-terminal human p53R2 from (aa 200-351).
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol and 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
p53R2, a crucial player in cellular survival, operates in a p53/TP53-dependent manner to facilitate the repair of damaged DNA. It assumes a vital role by providing deoxyribonucleotides essential for DNA repair processes, particularly in cells arrested at G1 or G2 phases. The protein harbors an iron-tyrosyl free radical center, indispensable for its catalytic function. Notably, p53R2 forms an active ribonucleotide reductase (RNR) complex in conjunction with RRM1. This complex is expressed in both resting and proliferating cells, responding adeptly to DNA damage, highlighting its significance in maintaining genomic integrity.
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Subcellular Localization
Cytoplasm, Nucleus
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Expression
Tissue specificity: Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
Induction: In response to DNA damage in a wild-type p53/TP53-dependent manner. -
Isoforms & Post-Translational Modification
p53R2 has 6 isomers: Q7LG56-1: 351 amino acids, molecular weight 40,737 Da (predicted); Q7LG56-2: 299 amino acids, molecular weight 34,529 Da (predicted); Q7LG56-3: 139 amino acids, molecular weight 15,963 Da (predicted); Q7LG56-4: 66 amino acids, molecular weight 7,602 Da (predicted); Q7LG56-5: 43 amino acids, molecular weight 4,852 Da (predicted); Q7LG56-6: 423 amino acids, molecular weight 48,787 Da (predicted).
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Subunit
Heterotetramer with large (RRM1) subunit. Interacts with p53/TP53. Interacts with RRM1 in response to DNA damage.
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SwissProt ID
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Synonyms
P53R2, RRM2B, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2
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