p73 Antibody (YA1240)(PBS only)

Cat. No.: HY-P81495A: Data Sheet User Guide for Antibodies
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p73 Antibody (YA1240) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p73.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

p73 Antibody (YA1240) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p73.

Host

Rabbit

Molecular Weight

Predicted band size: 70 kDa;

Observed band size: 70/60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O15350

Gene ID

7161 [NCBI]

Immunogen

Recombinant protein of human p73

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression (By similarity). It is an essential factor for the positive regulation of lung ciliated cell differentiation (PubMed:34077761)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Expressed in striatal neurons with Huntington disease (at protein level) . Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. Expressed in the respiratory epithelium (PubMed:34077761)

Induction:Not induced by DNA damage. Isoforms lacking the transactivation domain block gene induction

Isoforms & Post-Translational Modification：O15350 has 12 isomers: O15350-1: 69623 Da (predicted); O15350-2: 54322 Da (predicted); O15350-3: 53026 Da (predicted); O15350-4: 44517 Da (predicted); O15350-5: 61694 Da (predicted); O15350-6: 59887 Da (predicted); O15350-8: 64422 Da (predicted); O15350-9: 49121 Da (predicted); O15350-10: 47824 Da (predicted); O15350-11: 62034 Da (predicted); O15350-12: 38874 Da (predicted); O15350-13: 61694 Da (predicted).
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function;Higher levels of phosphorylation seen in the brain from patients with Huntington disease;Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome

Subunit：Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation. Interacts (via SAM domain) with NQO1; this interaction is NADH-dependent, stabilizes TP73 in response to oxidative stress and protects it from ubiquitin-independent degradation by the 20S proteasome

Research Field

Cell Biology

Synonyms

TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein

Documentation

User Guide for Antibodies (1077 KB)