PARG Antibody (YA7853)(PBS only)

Cat. No.: HY-P88169A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PARG Antibody (YA7853) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PARG.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PARG Antibody (YA7853) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PARG.

Host

Mouse

Molecular Weight

Predicted band size: 110.9 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q86W56

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-367 of human PARG produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34019811, PubMed:34321462). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521). Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811). Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880). Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521). Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters (PubMed:23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257)

Subcellular Localization：Nucleus,Cytoplasm,Cytoplasm,Cytoplasm,Mitochondrion,Mitochondrion matrix

Expression：
Tissue_Specificity: Ubiquitously expressed

Isoforms & Post-Translational Modification：Q86W56 has 5 isomers: Q86W56-1: 111110 Da (predicted); Q86W56-2: 102312 Da (predicted); Q86W56-3: 99432 Da (predicted); Q86W56-4: 60873 Da (predicted); Q86W56-5: 54794 Da (predicted).

Subunit：Interacts with PCNA (PubMed:21398629)

Synonyms

PARG99

Documentation

User Guide for Antibodies (1077 KB)