PTPN22 Antibody (YA8394)

Cat. No.: HY-P88710: Data Sheet User Guide for Antibodies
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PTPN22 Antibody (YA8394) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PTPN22.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PTPN22 Antibody (YA8394) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PTPN22.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 91.5 kDa

Species Reactivity

Human

SwissProt ID

Q9Y2R2

Immunogen

Human recombinant protein fragment corresponding to amino acids 359-449 of human PTPN22 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:800-1500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules (PubMed:16461343, PubMed:18056643). Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue (PubMed:21719704). Dephosphorylates ZAP70 at its activating 'Tyr-493' residue (PubMed:16461343). Dephosphorylates the immune system activator SKAP2 (PubMed:21719704). Positively regulates toll-like receptor (TLR)-induced type 1 interferon production (PubMed:23871208). Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy (PubMed:23991106). Acts as an activator of NLRP3 inflammasome assembly by mediating dephosphorylation of 'Tyr-861' of NLRP3 (PubMed:27043286). Dephosphorylates phospho-anandamide (p-AEA), an endocannabinoid to anandamide (also called N-arachidonoylethanolamide) (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils (PubMed:15208781). Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells
Induction: By muramyl-dipeptide and lipopolysaccharide

Isoforms & Post-Translational Modification：Q9Y2R2 has 6 isomers: Q9Y2R2-1: 91705 Da (predicted); Q9Y2R2-2: 78769 Da (predicted); Q9Y2R2-3: 85138 Da (predicted); Q9Y2R2-4: 88647 Da (predicted); Q9Y2R2-5: 20932 Da (predicted); Q9Y2R2-6: 75488 Da (predicted).
Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases

Subunit：Interacts with CSK (PubMed:15208781)

Synonyms

LYP; LYP1; LYP2; PEP; PTPN8

Documentation

User Guide for Antibodies (1077 KB)