RASSF1A Antibody (YA7255)

Cat. No.: HY-P87571: Data Sheet User Guide for Antibodies
FAQs
Technical Support

RASSF1A Antibody (YA7255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RASSF1A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

RASSF1A Antibody (YA7255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RASSF1A.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 39 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NS23

Immunogen

A synthesized peptide derived from human RASSF1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage

Subcellular Localization：Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm, cytoskeleton, spindle,Cytoplasm, cytoskeleton, spindle pole,Nucleus,Nucleus

Expression：
Tissue_Specificity: Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells

Isoforms & Post-Translational Modification：Q9NS23 has 8 isomers: Q9NS23-1: 39219 Da (predicted); Q9NS23-2: 38814 Da (predicted); Q9NS23-3: 21899 Da (predicted); Q9NS23-4: 31226 Da (predicted); Q9NS23-5: 39265 Da (predicted); Q9NS23-6: 9937 Da (predicted); Q9NS23-7: 16120 Da (predicted); Q9NS23-9: 8656 Da (predicted).

Subunit：Interacts with MAP1S (PubMed:15205320, PubMed:15753381)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]