RSPO3 Antibody (YA7994)

Cat. No.: HY-P88310: Data Sheet User Guide for Antibodies
FAQs
Technical Support

RSPO3 Antibody (YA7994) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RSPO3.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RSPO3 Antibody (YA7994) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RSPO3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 30.7 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9BXY4

Immunogen

Human recombinant protein fragment corresponding to amino acids 22-272 of human RSPO3 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed:21727895, PubMed:21909076, PubMed:22615920). Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (By similarity) (PubMed:21727895, PubMed:21909076, PubMed:22615920). Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3 (PubMed:29769720)

Subcellular Localization：Secreted

Expression：
Tissue_Specificity: Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node (PubMed:12463421). Highly expressed in endothelial cells (PubMed:26766444)

Isoforms & Post-Translational Modification：Q9BXY4 has two isomers: Q9BXY4-1: 30929 Da (predicted); Q9BXY4-2: 33233 Da (predicted).

Subunit：Interacts with the extracellular domain of FZD8 and LRP6 (By similarity)

Synonyms

CRISTIN1; PWTSR; THSD2

Documentation

User Guide for Antibodies (1077 KB)