S100A12 Antibody (YA7780)(PBS only)
(Synonyms: CAAF1; CAGC; CGRP; ENRAGE; MRP-6; MRP6; p6)S100A12 Antibody (YA7780) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to S100A12.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:150-500 |
Product Details
S100A12 Antibody (YA7780) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to S100A12.
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Host Mouse
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 10.4 kDa
Full length human recombinant protein of human S100A12 produced in HEK293T cell.
Affinity purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
S100A12 is a calcium-binding S100/calgranulin protein secreted by activated neutrophils and granulocytes, with proinflammatory activity centered on interaction with RAGE[1][2]. Mechanistically, S100A12-RAGE engagement activates NF-κB-dependent endothelial and inflammatory signaling, supporting cytokine production, adhesion biology, and chronic cellular activation[2][3]. In disease models and human inflammatory samples, S100A12 is strongly expressed in active inflammatory bowel disease and inflamed synovial tissue, and circulating levels correlate with disease activity[1][2]. Compared with related S100/calgranulin isoforms, S100A12 has a key experimental distinction because functional S100A12 is absent from the murine genome, limiting direct interpretation of standard mouse models[4]. For experimental applications, structural studies show that S100A12 binds the RAGE V domain, while tranilast and FPS-ZM1 reduced S100A12-induced cellular responses in vitro[5].
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Subcellular Localization
Secreted,Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
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Expression
Tissue_Specificity: Predominantly expressed by neutrophils, monocytes and activated macrophages. Expressed by eosinophils and macrophages in asthmatic airways in regions where mast cells accumulate. Found in high concentrations in the serum of patients suffering from various inflammatory disorders, such as rheumatoid arthritis, psoriatic arthritis, Crohn's disease, ulcerative colitis, and Kawasaki disease -
Isoforms & Post-Translational Modification
P80511: 92 amino acids, molecular weight 10575 Da.
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Subunit
Homodimer
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SwissProt ID
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Synonyms
CAAF1; CAGC; CGRP; ENRAGE; MRP-6; MRP6; p6
Documentation
References
[1]. Foell D, et al. Neutrophil derived human S100A12 (EN-RAGE) is strongly expressed during chronic active inflammatory bowel disease. Gut. 2003 Jun;52(6):847-53. [Content Brief]
[2]. Foell D, et al. Expression of the pro-inflammatory protein S100A12 (EN-RAGE) in rheumatoid and psoriatic arthritis. Rheumatology (Oxford). 2003 Nov;42(11):1383-9. [Content Brief]
[3]. Hofmann MA, et al. RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell. 1999 Jun 25;97(7):889-901. [Content Brief]
[4]. Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol. 2003 Dec;24(12):619-22. doi: 10.1016/j.it.2003.10.001. PMID: 14644131. et al. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol. 2003 Dec;24(12):619-22. [Content Brief]
[5]. Chiou JW, et al. Blocking the Interactions between Calcium-Bound S100A12 Protein and the V Domain of RAGE Using Tranilast. PLoS One. 2016 Sep 6;11(9):e0162000. [Content Brief]
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