SF3A1 Antibody (YA8078)

Cat. No.: HY-P88394: Data Sheet User Guide for Antibodies
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SF3A1 Antibody (YA8078) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SF3A1.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SF3A1 Antibody (YA8078) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SF3A1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 88.7 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15459

Immunogen

Human recombinant protein fragment corresponding to amino acids 249-568 of human SF3A1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:11533230, PubMed:32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:10882114, PubMed:11533230, PubMed:32494006). Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed:10882114, PubMed:11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes (PubMed:29360106, PubMed:30315277)

Subcellular Localization：Nucleus,Nucleus speckle

Expression：
Tissue_Specificity: Ubiquitously expressed

Isoforms & Post-Translational Modification：Q15459 has two isomers: Q15459-1: 88886 Da (predicted); Q15459-2: 81678 Da (predicted).

Subunit：Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins (PubMed:32494006, PubMed:36797247)

Synonyms

PRP21; PRPF21; SAP114; SF3A120

Documentation

User Guide for Antibodies (1077 KB)