SF3B14 Antibody (YA8273)
(Synonyms: CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a)SF3B14 Antibody (YA8273) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SF3B14.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:800-1500 |
Product Details
SF3B14 Antibody (YA8273) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SF3B14.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 14.4 kDa
Full length human recombiant protein of human SF3B14 produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SF3B14 component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B6 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Within the 17S U2 SnRNP complex, SF3B6 directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. SF3B6 stabilizes the intron branch site-U2 snRNA duplex, thereby promoting-binding of introns with poor sequence complementarity. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
Q9Y3B4: 125 amino acids, molecular weight 14585 Da.
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Subunit
Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins (PubMed:12234937, PubMed:15146077, PubMed:32494006, PubMed:34822310)
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SwissProt ID
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Synonyms
CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a
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