SHMT2 Antibody (YA7793)(PBS only)

Cat. No.: HY-P88109A: Data Sheet User Guide for Antibodies
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SHMT2 Antibody (YA7793) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SHMT2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SHMT2 Antibody (YA7793) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SHMT2.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P34897

Immunogen

Full length human recombinant protein of human SHMT2 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:25619277, PubMed:29364879, PubMed:33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29364879, PubMed:29452640). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985)

Subcellular Localization：Mitochondrion,Mitochondrion matrix, mitochondrion nucleoid,Mitochondrion inner membrane,Cytoplasm,Nucleus

Isoforms & Post-Translational Modification：P34897 has three isomers: P34897-1: 55993 Da (predicted); P34897-2: 54863 Da (predicted); P34897-3: 53455 Da (predicted).
Succinylation at Lys-280 inhibits the hydroxymethyltransferase activity

Subunit：Homotetramer; in the presence of bound pyridoxal 5'-phosphate (PubMed:25619277, PubMed:29180469)

Synonyms

GLYA; HEL-S-51e; NEDCASB; SHMT

Documentation

User Guide for Antibodies (1077 KB)